一株高效高温菌的筛选初步鉴定和性质研究.pdfVIP

第 6 期 华东师藏大学学报(自然科学版) No.6 20()H 文章编号: 1000-5641 (2008)闲时0082翩06 一株高效高温菌的筛选初步鉴定和性质研究 秦 蓉1 ， 李秀艳1 ， 李华芝2 ， 韩波波1 ， 黄民生1 (1.华东师范大学环境科学系，上海 2000621 2. 上海新东京(集团)有限公司，上海 200232) 摘要:从高温堆肥物料中通过平板培养法分离、纯化并筛选出一株高效高温菌 YBI6. Y日16 具 有间时分解淀粉、盖白质、汹脂和纤维素等大分子有饥物的能力.经鉴定 YB16 为革兰氏阳性， 轩状，有穿抱，好氧.G+Cmol% 为50.66% ，初步鉴定 YB16 属于芽抱忏菌鹰. pH 生长范围为 6.0-9.0 ，最适生长温度为 65 C 左右. 关键诩:高温IW; 鉴定$ 芽抱杆菌p 降解能力1 G+Cmol% 中图分类号: Q 948 文民标识码:A Screening , identification and properties of a strain of thermophilic bacterium with strong degradation ability QIN Rong , LI Xiu-yan 1, LI Hua-zhî2 , HAN Bo-bo , HUANG Min-吩. (门1. Departrnent οf En唱 VI旷Ironmen时It Sc口2附附e肝enc时e.E，αstC泊hín阳α Nο旷俨m负m阳aωi 【Univer川sitηy. Shanghai 200062. Chin阳10们 $ 2. Shanghai N I!W Tokyo Group .Shanghai 200232. China) Abstract , A straín of thermophilíc bacteríum with strong degradation ability named YB16 was plat e- screened from thermophilic compost materials. 丁he se!ected strain can decompose organic macromolecules such as starch , protein , lipid and cellulose simultaneous!y. YB16 was aerobic , Gram positive , rod , and formed spore and capsule. 丁he results of DNA UV absorbency showed that G + C mol% of YB16 was about 50.66%. It was consídered to be a variety of Bacillus sp. YB 16 grew well at pH 6. 0-9. O. The optimal growth temperature was about 65 C. Key words: thermophilic bacteria; identification; Bacillus sp. 1 degradatíon ability; G+ C mol% o ~I 宫 荧国人 Thomas Brock 于 1969 年首先从荧国黄石国家公回分离出第…个严格意义的 收稿日期:2