以丙氨酸消旋酶为靶标的中药抗菌成分的初步筛选.pdfVIP

.966. 现代医药卫生 2013年4月 15 日 第29卷第7 期 J Mod Med Health.April 15 ,2013 ,Vo I.29 ,No.7 ·论著· 以丙氨酸消旋酶为靶标的中药抗菌成分的初步筛选 王敏兰，石亚伟(山西大学生物技术研究所/教育部化学生物学与分子工程重点实验室，山西太原 030006) {摘 要] 目的 从 89 种具有抗菌、消炎作用的药材中筛选能够抑制丙氨酸消旋酶活性的中药。方法 利用旋光 仪测定丙氨酸消旋酶在有无中药材浸出物情况下酶活性从而对 89 种中药进行初步筛选1 结果 89 种中药中有 25 种能 够不同程度地抑制丙氨酸消旋酶活性，其中 6 种具有明显的抑制作用(抑制率大于 35%) 。结论 虎杖、黄苓、麻黄、吴某 笑、梳子、鸡尿藤 6 种中药能明显抑帝11玛氨酸消旋酶活性。 [关键词] 丙氨酸外消旋酶; 中草药; 抗菌; 抑制剂; 筛选 doi: 1O.3969/j.issn.1009-5519.2013.07.003 文献标识码:A 文章编号: 1009-5519(2013) 07 -0966-02 Primary sreening of antibacteriaI components with aIanine racemase as target in traditional Chinese medicines Wang Minlan ,Shi Yawei(lnstitute 0/Biotechnology ,Key Lαborωory， 0/ Chemical Bωlogy and Molecular Engineering 0/Educalion Min- istry ,Shanxi Universi妙，r.ι叩剧， Shanxi 030006 , China) [Abstract] Objective To screen the trad山onal Chinese medicines (TCM) with ihe effects inhibiting alanine racemase activity from 89 kinds of medicinal materials possessing antibacterial role. Methods 89 kinds of traditional Chinese medicines were primarily screened by using polarimeter to determine the enzymatic activity with or without the extraction of traditional Chi- nese medicine. Results 25 kinds of traditional Chinese medicines from 89 kinds of TCM could inhibit the alanine racemase ac- tivities in different degrees ,and 6 kinds had obvious inhibiting role (inhibiting rate35%). Conclusion Polygonum Cuspidatum , Baikal Skullcap Root ,Ephedra Herb ,Medicinal Evodia Fruit , Cape Jasmine and Chinese Fevervine Herb can significantly inhibit the alanine racemase activity. [Key words] Alanine racemase; Orugs ,chinese herbal; Antibiosis; Inhibitor; Screen 抗菌药物的滥用导致病菌耐药性增强，加之新型抗菌药物的