Glucocorticoidaffectsdendritic.PDF

/scientificreports OPEN Glucocorticoid affects dendritic transport of BDNF-containing vesicles Received: 26 November 2014 1,2,3 1,2,4 1,5 6 Accepted: 06 July 2015 Naoki Adachi , Tadahiro Numakawa , Shingo Nakajima , Masashi Fukuoka , 1,7 1,7 1,6 6 Haruki Odaka , Yusuke Katanuma , Yoshiko Ooshima , Hirohiko Hohjoh Published: 04 August 2015 Hiroshi Kunugi1,2 Brain-derived neurotrophic factor (BDNF) is essential for neuronal survival, differentiation, and functions in the central nervous system (CNS). Because BDNF protein is sorted into secretory vesicles at the trans-Golgi network in the cell body after translation, transport of BDNF-containing vesicles to the secretion sites is an important process for its function. Here we examined the effect of dexamethasone (DEX), a synthetic glucocorticoid, on BDNF-containing vesicle transport and found that DEX decreased the proportion of stationary vesicles and increased velocity of the microtubule- based vesicle transport in dendrites of cortical neurons. Furthermore, DEX increased huntingtin (Htt) protein le