栀子不同外植体愈伤诱导效果比较分析.pdfVIP

8 东南园艺 2016年第4期 栀子不同外植体愈伤诱导效果比较分析 高敏霞韦晓霞+ (福建省农业科学院果树研究所，福建福州350013) 摘 要：以MS培养基为基本培养基，将栀子叶片、茎段、幼嫩花苞、未成熟果实的子房壁接种于不同成分的诱 导培养基上，研究不同外植体愈伤的诱导情况。结果表明：不同外植体的诱导效果存在显著差异，叶片的诱导率 0．5 0．02 最高，其次是花苞、子房壁，茎段的诱导率最低；最佳愈伤组织诱导培养基为MS+6-BAmg·L．。+TDZ mg·L～，激素TDZ的使用有利于提高愈伤诱导率并改善愈伤质地。叶片诱导率高，且获得的愈伤质地较疏松， 呈颗粒状，适合进一步培养和诱导分化。 关键词：栀子；外植体；愈伤；诱导；比较分析 EffectsofDifferent on Ellis CallusInductionofGardenia Explants jasminoides GA0 Min—xiaWEIXiao—xia+ Research (Fruit Institute，FujianAcademyofAgriculturalSciences，眦u350013，FujianChina) MSmediumasbasic wallofimmaturefruitofGardenia Abstract：In medium，leaf，stem segment，youngbuds，ovary Elliswereinoculatedondifferent ofinductionmediato theinductionofcallusfromdifferent jasminoides components study were inductionrateof show：There differencesintheinductioneffectsofdifferent explants．Results significant explants，the leavesWas followedbudsand wallandtheinductionrateofstemsectionislowest．Themediumfor hiIghest by ovary optimal callusinductionWasMS+6一BA of 0．5 0．02 usehormoneTDZWasbeneficialto the mg·L～+TDZ improve mg·L～．The callusinductionrateandthe ofthecallus．The ofleafwas itscalluswas