武夷山及周边地区黄精植物ISSR分子标记鉴定及HPLC指纹图谱研究.pdfVIP

2017,46(1): 25 ～29. Subtropical Plant Science 武夷山及周边地区黄精植物 ISSR 分子标记鉴定 及 HPLC 指纹图谱研究 1 2 2 2 1 杨 青 ，高华春 ，陈红丽 ，陈全成 ，徐鲜钧 (1.武夷山生物研究所，福建 武夷山 354300 ；2.厦门大学药学院，福建 厦门 361102) 摘 要：采用 ISSR 分子标记鉴定方法及 HPLC 色谱方法对武夷山及周边地区 5 份黄精植物样本进行 DNA 分 子标记鉴定及指纹图谱分析，并通过聚类分析软件分别探讨其遗传相关聚类图谱。ISSR 分子标记法能较好地 对 5 份黄精植物样本进行区分，HPLC 指纹图谱分析同样表明 5 份黄精样本化学成分存在一定的差别。DNA 分子标记及 HPLC 指纹图谱分析的遗传相关聚类分析结果相似，表明武夷山及周边地区的野生黄精种质资源存 在明显的差别。 关键词：黄精；ISSR 分子标记；HPLC 指纹图谱 Doi: 10.3969/j.issn.1009-7791.2017.01.005 中图分类号：Q949.95 文献标识码：A 文章编号：1009-7791(2017)01-0025-05 Identification of Polygonatum from Wuyishan and Its Surrounding Areas by ISSR Molecular Markers and Their HPLC Fingerprints 1 2 2 2 1 YANG Qing , GAO Hua-chun , CHEN Hong-li , CHEN Quan-cheng , XU Xian-jun (1.Wuyishan Institute of Biology, Wuyishan 354300, Fujian China; 2.School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, Fujian China) Abstract: The purpose of this project was to identify and analyze the genetic relationships of 5 kinds of Polygonatum in Wuyishan and its surrounding areas. Using ISSR marker and HPLC molecular identification method for chromatographic methods analysis of DNA markers for identification and fingerprinting of 5 Polygonatum samples, the genetic correlation map of Polygonatum was studied by cluster analysis software. ISSR molecular marker method could be used to distinguish Polygonatum of Wuyishan and its surrou