2015-(图片质量好,有参考价值)两种方法制备的脑组织切片激光共聚焦显微镜成像效果的比较研究_李贵苹.pdf

Chinese Journal of Cell Biology 2015, 37(11): 1528–1533 DOI: 10.11844/cjcb.2015.11.0196 1 ( , 200025; 2 , 200025) OCT , , , , , , , , ; ; ; Comparison of Vibratome and Frozen Sections of Brain Tissue in the Quality and Image of Confocal Laser Scanning Microscopy 1 2 1 1* Li Guiping , Zhu Xiaona , Yi Jing , Hang Qin 1 ( Department of Biochemistry and Molecular Cell Biology, Shanghai Jiaotong University 2 School of Medicine, Shanghai 200025, China; Neuroscience Division, Department of Anatomy, Histology and Embryology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China) Abstract We studied the quality of vibratome sections and frozen sections of brain tissues and evaluated the imaging quality of these two sections with laser scanning confocal microscope (LSCM), respectively. Brain tissues of 6- to 8-week-old mice (CD1) were fi xed by perfusion and then embedded in low-melting agarose, sliced on a vibratome, or embedded in OCT compound, sliced on a freezing microtome. The vibratome sections and the frozen sections were demonstrated by immunofl uorescence staining and observed by confocal laser scanning microscopy. The results showed that compared with frozen sections, vibratome sections of brain tissues offered distinct advantages: easier embedding approaches, higher quality of brain tissues sections, better structural integrity of tissue sections, and superior image quality of confocal laser scanning microscopy. Keywords brain tissues; vibratome s