生物信息名词介绍及报告解读-1.pdf

生物信息学 名词介绍及报告解读 上海丰核 021 一、名词介绍 I. About Microarray, Gene expression profiling Data preprocessing and differentially expressed genes analysis II. About network , clique, module , common target gene III. About pathway, process, function a otatio … IV. Special bioinformation vocabulary V. Statistical vocabulary 上海丰核 021 PART 1 Gene expression profiling: In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell. 上海丰核 021 PART 1  R language:  R is an open source programming language and software environment for statistical computing and graphics. The R language is widely used among statisticians and data miners for developing statistical software and data analysis.  LIMMA:  Data analysis, linear models and differential expression for microarray data I. T-test II. 贝叶斯矫正 上海丰核 021 PART 1 Gene Expression Omnibus (GEO) : a database repository of high throughput gene expression data and hybridization arrays, chips, microarrays. 上海丰核 021 PART 1 Example We extracted the gene expression profile data on prostate cancer patients with normal controls from which were deposited in NCBI GEO (/geo/) database (ID: GSE3325) based on the Affymetrix Human Genome U133 plus 2.0 Array. Total 19 chips were available for further analysis, including 13 individual benign pro