辣木蛋白酶及其凝乳机理的研究(修改).doc

辣木蛋白酶及其凝乳、净水机理研究 The mechanism of milk-clotting and water purification from Morlnga oleifera protease 中文摘要（限400字） 根据前期实验和相关报道，辣木花、叶、籽均含有蛋白酶，且能凝乳，特别是辣木籽的净水功能声名远扬，但未见其深入研究。采用水浸泡、真空浓缩、冷冻干燥以及SDS电泳分离蛋白酶、超滤纯化、ESI质谱酶解肽段α-酪蛋白、-酪蛋白和β-酪蛋白κ-酪蛋白的酶切位点等一级结构变化及产物，揭示辣木蛋白酶的凝乳机理。根据电泳条带、HPLC多肽图谱、FAA等的变化研究辣木籽蛋白酶对污水大分子蛋白质的降解作用；利用扫描电镜、传输扩散波光谱The protease and milk coagulating, water purification properties were found from Morlnga oleifera flower, leaves and seeds according to the former test. Extraction, isolation and identification of the protease from Morlnga oleifera flower, leaves and seeds is going to be developed by the steps of bath, vacuum concentration, freeze-drying, separation, ultrafiltration and mass spectrum. The milk-clotting mechanism of the protease will be researched according to the cleavage site on κ-casein and Casein degradation by SDS，matrix-assisted laser desorption time-of-flight mass and HPLC during coagulation. The water purification mechanism of the protease from Morlnga oleifera seeds is about to be determined according to the sewage protein degradation and the association, flocculating reaction, microscopic structure change by the methods of SDS，HPLC, Confocal Scanning Laser Microscopy, UV spectrum and Diffusing Wave Spectroscopy, et al. The scientific theory of milk-clotting and water purification mechanism from Morlnga oleifera protease is important to develop new chymosin and enzyme engineering purifying agent and benefit to supplement the scientific system of Morlnga oleifera. 关键词：辣木蛋白酶Morlnga oleifera protease；蛋白降解protein degradation；凝乳机理milk-clotting mechanism；电离飞行质谱matrix-assisted laser desorption time-of-flight mass；κ-酪蛋白酶切位点the cleavage site on κ-casein；絮凝反应flocculation reaction；water purification mechanism； 二、报告正文：参照以下提纲撰写，要求内容翔实、清晰，层次分明，标题突出。 （一）立项依据与研究内容（4000-8000字）： 1、项目的立项依据（研究意义、国内外研究现状及发展动态分析，需结合科学研究发展趋势来论述科学意义；或结合国民经济和社会发展中迫切需要解决的关键科技问题来论述其应用前景。附主要参考文献目录） （1）辣木简介 辣木(Morlnga oleifera Lain.)为辣木科辣木属多年生热带落叶乔木，全世界约有14个品种，广泛种植于亚洲和非洲热带和亚热带地区，生长快、耐干