LncRNA专题.pdf

LncRNA LncRNA ——Dark Matter of the genome are gradually coming to light ——Dark Matter of the genome are gradually coming to light Nonconding RNA 93% transcripts housekeeping noncoding RNAs :tRNA、rRNA 、 snRNA 、snoRNA regulatory noncoding RNAs: 1、short regulatory noncodingRNAs: microRNAs, small interfering RNAs and Piwi-associated RNAs 2 、lncRNAs 2009,Cell, Chris P. Ponting Definitions Long non-protein-coding RNA, or lncRNA ( lncRNAs): Any endogenous cellular Long non-protein-coding RNA, or lncRNA ( lncRNAs) RNA that is mRNA-like in length Lacking any positive-strand open reading frames greater than100 amino acids in length Supported by experimental evidence and distinct from any known functional- RNA classes (including but notlimited to ribosomal, transfer, and small nuclear/nucleolar RNAs) long RNA, large RNA, macro RNA, intergenic RNA, NonCoding Transcripts, or other synonyms 2009, Cell 136(4),C.P. Ponting, P.L. Oliver, W . Identification of long ncRNAs longer than ~200 nucleotides has little or no protein-coding capacity lack satisfactory classifications roles in transcriptional regulation, post-transcriptional regulation ,epigenetic gene regulation, and disease 2009,Nature Reviews Tim R. Mercer Classifications Nature Antisense Intronic antisense bidirectional Exon-sense Overlapping intergenic Intron-sense LincRNA(large intergenic) LncRNA with Enhance-like …… /supports/support_main.asp Functions of long ncRNAs Chromatin modification Transcriptional regulation Post-transcriptional regulation Chromatin modification long ncRNAs can mediate epigenetic changes by recruiting chromatin remodelling complexes to specific genomic l