目的基因获取的策略与方法.pdf

目的基因获取的策略与方法 The Strategy and Methods to obtain the Gene of Interest 王 方 教授 王 方 教授 基础医学院 神经生物学教研室 基础医学院 神经生物学教研室 Oct.11,2007 Oct.11,2007 Main Contents in the Topic 1. Outline and Background. 2. To obtain an interested gene with unknown background. 3. To obtain the gene of interest from only knowing a piece of related nucleotide sequence. 4. To obtain the interested gene initiated from the information of a protein/peptide. 5. To obtain a chimeric gene for creating a fusion Pr. 6. What should be done after a novel gene is obtained? International sequence databases exceed 100 gigabases GenBank Database CoreNucleotide contains all Nucleotide records that are not in EST or GSS EST contains only Expressed Sequence Tag records. GSS contains only Genome Survey Sequence records. GenBank INSDC (International Nucleotide Sequence Database Collaboration) EMBL DDBJ exchange data daily 基因操作相关简史 1944年, Macleod和Mc Carty发现并证明DNA 是生物遗传的信息分子. 1953年, Watson和Crick 发现DNA 的双螺旋结构并提出了遗传的中心法则 (The central dogma ), 开创了分子生物学的新