菊苣根中多糖的提取工艺本科论文 .doc

摘要 本以为原料L9(34) 正交℃，料液比1:20，提取时间为8h，在此最优条件下，菊苣根多糖的提取率达到6.426%。方法学考察表明，苯酚硫酸法在测定菊苣根多糖含量时，方法简便，结果准确，重现性好，稳定性好。 关键词：；Abstract Chicory, the paper as raw material, after extraction, centrifugation and filtration, the preparation of chicory root polysaccharide in addition to protein concentration, and alcohol precipitation process, test analysis and comparison of the hot water bath extraction, alcohol bath extraction, ultrasonic water extraction and ultrasonic ethanol extraction four methods the effect of the extract of chicory root polysaccharide, polysaccharides, and chicory root extract was investigated object by single factor test analysis, the effects of extraction temperature, time, solid-liquid ratio of polysaccharide extraction rate to determine the Sevag method in addition to protein chloroform: butanol ratio of 3:1 (v: v), and alcohol precipitation of solid to liquid ratio of 2:1 (v: v), L9(34)orthogonal experiments to determine the optimum conditions for extraction of the hot water bath polysaccharide extract of chicory root as follows: extraction temperature of 80 ° C, the ratio 1:20, extraction time of 8h, the optimal conditions, the polysaccharide of chicory root extract rate of 6.006%. Methodological study showed that the phenol sulfuric acid method in the determination of Chicory root polysaccharide content, the method is simple, accurate with good reproducibility, good stability. Key words: Chicory root polysaccharide；polysoccharide；extract；orthogonal test； 目录 摘 要 I Abstract II 第一章 前 言 1 第二章 文献综述 1 2.1 菊苣根研究现状 1 2.2 菊苣根的功能 2 2.2.1 抗衰老作用 2 2.2.2 调节心脑血管的功能 2 2.2.3 镇静作用 2 2.2.4 抗肿瘤作用 2 2.3 菊苣根中几种活性成分 3 2.3.1 菊苣根中微量元素及氨基酸 3 2.3.2菊苣根中其他成分 3 2.3.3菊苣根多糖 3 2.4 多糖的几种提取方法 6 2.4.1热水浸提法 6 2.4.2 酸浸提发 6 2.4.3 超声波提取法 6 2.4.4 超临界萃取法 7 2.5 研究意义 7 第三章 实验部分 8 3.1原料与设备 8 3.1.1 原料 8 3.1.2试剂 8 3.2检测方法 9 3.2.1苯酚-硫酸法测定多糖含量 9 3.3菊苣根多糖的提取方法研究 10 3.3.1菊苣根的预处理 10 3.3.2菊苣根多糖提取方法的比较 10 3.4 菊苣根多糖提取工艺的优化 11 3.4.1 菊苣根多糖的提取工艺 11 3.4.2 Sevag法去除菊苣根多糖中的蛋白 11 3.4.3乙醇浓度对多糖提取率的影响 11 3.4.4 菊