720min累积释放达96.81%和96.88%,在40min内.PDF

维 A 酸维生素 C 棕榈酸酯泡囊的体外释放、经皮渗透和皮肤贮留研究 陈正明，龙晓英* ，丁钢，袁飞(广东药学院中药学院，广州 510006) 摘要：目的 研究维生素 C 棕榈酸酯泡囊作为维 A 酸载体时，主药的体外释放、经皮渗透和皮肤贮留情况。方法 采用 Franz 扩散池测定维 A 酸从载体中的释放速度，扩散池与供给池之间为纤维素膜(截留分子量 8 000~14 000) ，扩散池面积 为 2.92 cm2 。体外透皮试验用小鼠、大鼠或兔子背部皮肤替代半透膜。在体外透皮试验完结后，取下皮肤，剪碎匀浆， 用 50%异丙醇-生理盐水提取，提取液处理后用 HPLC 测定药物浓度。结果 结果表明维 A 酸释放速率和累积经皮渗透量 大于市售乳膏，同时具有较高的皮肤贮留量。结论 维生素 C 棕榈酸酯泡囊作为维 A 酸载体有助于增加局部药物浓度。 作者简介：陈正明，男，硕士生，主管药师 Tel:E-mail: czmspu1012@ *通信作者：龙晓英，女，博士，教 授 Tel: (020E-mail: longxy3156@163.com 中国现代应用药学2010 年 6 月第 27 卷第 6 期 Chin JMAP, 2010 June, Vol.27 No.6 ·517 · 关键词：维 A 酸；维生素 C 棕榈酸酯；泡囊；体外释放；经皮渗透；皮肤贮留 中图分类号：R944.9 文献标志码：A 文章编号：1007-7693(2010)06-0517-06 Study on the Release Rate, in Vitro Skin Penetration and Retention of Tretinoin Ascorbyl Palmitate Vesicles * CHEN Zhengming, LONG Xiaoying , DING Gang, YUAN Fei(School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China) ABSTRACT: OBJECTIVE To study the release rate, in vitro skin penetration and retention of tretinoin when use ascorbyl palmitate vesicles as its carrier. METHODS The in vitro release rate of tretinoin from carrier was determined by Franz diffusion cell. Between donor compartment and receptor compartment was cellulose membrane(cut-off molecules 8 00014 000), the avaiable diffusion area of cell was 2.92 cm2 . In the in vitro skin penetration experiment, the back skin of mouse, rats or rabbits was used instead of cellulose membrane. After in vitro skin penetration experiment, the skin were taken down from cells, cut and homogenated, and then extracted by 50% isopropanol-normal saline. The extracting solution was analyzed by HPLC after treatment. RESULTS The res