草酸铵逆流萃取法提取西番莲果皮中果胶的研究.pdfVIP

226 2008, Vol.29, No.09 食品科学 ※工艺技术 黄永春，杨 锋，何 仁，程谦伟，乐伟平 (广西工学院生物与化学工程系，广西 柳州 545006) 摘 要： 对草酸铵逆流萃取法提取西番莲鲜果皮中的果胶进行研究，考察了逆流萃取的级数、温度、草酸铵浓 度、料液比和反应时间对果胶得率的影响，并对工艺条件进行了优化，获得草酸铵逆流萃取法提取西番莲果皮中 : 果胶的最优条件：逆流萃取级数为 3 级、温度 90 ℃、草酸铵浓度0.45% 、果皮质量与草酸铵体积比为 1 35 、提 取时间 5.5h 。与传统酸法提取相比，得率由2.22% 提高到 3.82% ，并对所制备果胶的品质进行测定。结果表明， 草酸铵逆流萃取法果胶得率、品质均优于传统酸法。 关键词： 果胶；西番莲果皮；草酸铵；逆流萃取法 Study on Countercurrent Extraction of Pecrin from Passiflora edulis Peel with Ammonium Oxalate HUANG Yong-chun ，YANG Feng ，HE Ren ，CHENG Qian-wei ，YUE Wei-ping (Department of Biological and Chemical Engineering, Guangxi University of Technology,Liuzhou 545006, China) Abstract ：Effects of countercurrent extraction stage, temperature, concentration of ammonium oxalate, material to solution ratio and extraction time on extraction rate of pectin from Passiflora edulis peel with ammonium oxalate were investigated in this study. The results showed that the optimum extraction conditions are as follows: countercurrent extraction stage 3, temperature 90 ℃; concentration of ammonium oxalate 0.35%; material to ammonium oxalate solution ratio 35:1 (g/ml) and extraction time 5.5 h. Compared with conventional method, the extraction rate of pectin increases from 2.22% to 3.82%. The results of quality determination of pectin showed that the method is high-performance, which does not change the quality of pectin. Key words ：pectin ；Passiflora edulis peel ；ammonium oxalate ；countercurrent extraction 中图分类号：TQ420.66 文献标识码：A 文章编号： 1002-6630(2008)09-0226-04 果胶是一种亲水性植物胶，广泛存在于绿色陆生植 随着功能性多糖