液体深层培养绿僵菌分生孢子地研究.pdf

第 37 卷 第 5 期 林 业 科 学 Vol37 , No5 　 　2 0 0 1 年 9 月 SCIENTIA SILVAE SINICAE Sep. , 2 0 0 1 液体深层培养绿僵菌分生孢子的研究 宋 　漳 (福建林学院资源与环境系　南平 353001) 关键词 : 　绿僵菌 , 液体深层培养 , 液生分生孢子 , 马尾松毛虫 , 毒力 STUDIES ON SPORULATION OF METARHIZIUM IN SUBMERGED CULTURE Song Zhang ( Resources Environment Department , Fujian College of Forestry 　Nanping 353001) Abstract : 　6 strains of Metarhizium were cultivated in submerged culture. The results indicated that the sub merged conidia could be produced by means of microcyclic sporogenesis for some strains of Metarhizium , but per haps , the occurrence of microcyclic sporogenesis depended on the nature of strains. Submerged sporulation of M337 strain was emphatically observed in the media containing different nutrient elements. The results showed that the submerged sporulation depended on a delicate equilibrium in the composition of the medium. Various carbon and ni trogen sources affected the production of submerged conidia greatly. Sucrose , soluble starch and lactose were effec tive for sporulation of submerged conidia. Peanut cake powder , yeast extract and peptone were ideal nitrogen sources for submerged sporulation. The influence of trace elements on submerged sporulation was most spectacular. Molyb denum was the most important element. Zinc was stimulatory. When all 6 elements (B ,Cu ,Fe ,Mn ,Mo ,Zn) were added together , it yielded highest production of submerged conidia. Vitamins affected submerged sporulation signifi cantly. VB6