小麦、蚕豆和油菜根系分泌物对麻花艽种子萌发影响.docVIP

小麦、蚕豆和油菜根系分泌物对麻花艽种子萌发的影响 　　摘要：分别用去离子水和乙酸乙酯萃取小麦、蚕豆和油菜根系分泌物刺激麻花艽（Gentiana straminea Maxim）种子，再进行麻花艽种子的发芽试验，考察小麦、蚕豆和油菜的根系分泌物对麻花艽种子萌发及胚根生长的影响。结果表明，小麦的根系分泌物水相浸提液对麻花艽种子萌发及胚根生长有轻微的促进作用，且随着水相浸提次数的增多，促进作用减弱。小麦的根系分泌物酯相浸提液对麻花艽种子萌发的影响不明显；蚕豆根系分泌物水相和酯相浸提液对麻花艽种子萌发及胚根生长抑制作用较弱；油菜根系分泌物水相和酯相浸提液对麻花艽种子萌发及胚根生长有明显的抑制作用，且随着水相和酯相浸提液浓度的降低，抑制作用减弱；油菜根系分泌物对麻花艽种子萌发及胚根生长的抑制作用最强，蚕豆根系分泌物对麻花艽种子萌发及胚根生长抑制作用较弱，小麦根系分泌物对麻花艽种子发芽有一定的促进作用 关键词：农作物；根系分泌物；麻花艽（Gentiana straminea Maxim）；萌发率；胚根长 中图分类号：S567.23；S154.36 文献标识码：A 文章编号：0439-8114（2016）14-3631-04 DOI：10.14088/j.cnki.issn0439-8114.2016.14.022 Abstract： In order to study the effect of wheat， broad bean， oilseed rape root extracts on germination and radical length of Gentiana straminea seeds， wheat， broad beans and oilseed rape root were extracted and partitioned by distilled water and ethyl acetate and arrange G. straminea seeds germinate text. Then the extract was subject to G. straminea seeds germination and radical growth. The results showed that wheat root aqueous phase extracts extracted by ethyl acetate had weak positive effect on G. straminea seeds germination and ridicle growth， with the increase of extraction times， the positive effect became weaken. Wheat root ester phase extracts extracted by ethyl acetate had unconspicuous effect. Broad bean root extracts had weak inhibitory effect； Oilseed rape had stronger inhibitory effect， with the increase of aqueous phase and ester phase extraction times， the positive effect became weaken； Oilseed root extracts had stronger inhibitory effect on germination and radical length of G. straminea seeds. Broad bean root extracts had weak inhibitory effect. Wheat root extracts had positive effect. Key words： crops； root exudates； Gentiana straminea Maxim； germination rate； radicle length 麻花艽（Gentiana straminea Maxim）属龙胆科龙胆属麻花艽组植物[1，2]，为《中华人民共和国药典》[3]记载的麻花艽主要品种之一，是一种常用的中藏药[1，4]，广泛分布于青海的大部分地区，四川的西北地区，甘肃、西藏、宁夏的部分地区[5]。由于制药需求，大量采集野生麻花艽，药源日渐耗竭，导致商品药材价格不断上涨，进一步加剧了野生种质资源的破坏[6，7]。人工种植技术成为既保护野生资源又保证市场供应，实