不同盐浓度对杨树组培苗抗氧化酶活力的影响.pdfVIP

安徽林业科技，2016 ，42（5 ）：17~20 42 卷6 期 Anhui Forestry Science and Technology 不同盐浓度对杨树组培苗抗氧化酶活力的影响 周思言 （响水县农业干部学校，江苏 盐城 224600 ） 摘 要：以NL-895 杨组培苗为材料，研究不同浓度的盐胁迫对其几种抗氧化酶活性的影响。结果表明，在50 mmol/L NaCl 处理下其具有较高的耐盐性，能够正常生长。在100 mmol/L NaCl 处理下受到一定的氧化胁迫，根对盐胁迫比叶 更敏感些；在100 mmol/L NaCl 盐胁迫10 d 时，根中AsA-POD 活性、GR 活性和叶中CAT 活性含量与对照相比，分别 上升了82.62%、36.36%、58.05% ；盐胁迫20 d 时，两种盐浓度处理下的根中CAT 活性变化幅度均大于叶。 关键词：杨树组培苗；盐胁迫；氧化胁迫；抗氧化酶；耐盐性 中图分类号：S792.11 文献标识码：A 文章编号：2095-0152（2016 ）06-0017-04 EffectsofDifferentSalinitiesontheAntioxidaseActivitiesof Poplar TissueCulturePlantlets ZHOU Siyan (Agricultural Cadre School of Xiangshui County, Yancheng 224600, Jiangsu, China) Abstract:In this paper tissue culture plantlets of poplar variety NL-895 were used as materials to study effects of different salinities on the antioxidase activities. The results showed that the plantlets treated with 50 mmol/L NaCl had relatively high salt tolerance and could grow normally. Those treated with 100 mmol/L NaCl suffered some oxidative stress with the roots being more sensitive to salt stress than the leaves; when salt stressed with 100 mmol/L NaCl for 10d, the active contents of AsA-POD and GR in the roots and the active contents of CAT in the leaves were 82.62%, 36.36% and 58.05% higher than those of the control group respectively; when salt-stressed for 20 d, the rangeabilities of CAT in the roots were higher than those in the leaves in the two salinities. Keywords:Poplar tissue culture plantlets; Salt stress; Oxidative stress; Antioxidase; Salt tolerance 杨树（Populus spp. ）是重要的造林绿化树种，也 抗氧化物质还原性所必须的酶类如谷胱甘肽还原