pcDNA6.2 nTC-Tag-DEST哺乳动物表达载体说明.pdf

pcDNA6.2/nTC-Tag-DEST 编号 载体名称 北京华越洋生物VECT6070 pcDNA6.2/nTC-Tag-DEST pcDNA6.2/nTC-Tag-DEST载体基本信息 载体名称: pcDNA6.2/nTC-Tag-DEST 质粒类型: 哺乳动物表达载体；cDNA 表达载体；报告载体；Gateway 载体 高拷贝/低拷贝: 高拷贝 克隆方法: Gateway 启动子: CMV 载体大小: 6796 bp 5 测序引物及序列: T7 Forward: 5’-TAATACGACTCACTATAGGG-3’ 3 测序引物及序列: TK polyA Reverse: 5’-CTTCCGTGTTTCAGTTAGC-3’ 载体标签: V5 Epitope(N-端）；TC tag(N-端） 载体抗性: 氨苄青霉素、氯霉素（仅空载体） 筛选标记: Blasticidin 克隆菌株: DB3.1 宿主细胞（系）: 常规细胞系，如293、Hela等 pcDNA6.2/cTC-Tag-DEST载体是cDNA 的表达与克隆载体； CMV 启动子驱动目的基因的过表达； TC Tag 即tetracysteine 备注: 基序是一段六氨基酸残基的肽段(Cys-Cys-Pro-Gly-Cys-Cys) ； 对应的检测试剂为FlAsH-EDT2和ReAsH-EDT2 ，既可以进行体内检测又可以 在胶内直接检测，高效灵敏。 稳定性: 瞬表达或稳表达 组成型/诱导型: 组成型 病毒/ 非病毒: 非病毒 pcDNA6.2/nTC-Tag-DEST载体质粒图谱和多克隆位点信息 pcDNA6.2/nTC-Tag-DEST 载体含有以下元件: Human cytomegalovirus immediate-early (CMV) promoter/enhancer for high-level expression in a wide range of mammalian cells TC-Tag for C-terminal (pcDNA6.2/cTC-Tag-DEST) or N-terminal (pcDNA6.2/nTC-Tag-DEST) fusion to the gene of interest for fluorescence detection Two recombination sites, attR1 and attR2, downstream of the CMV promoter for recombinational cloning of the gene of interest from an entry clone Chloramphenicol resistance gene located between the two attR sites for counterselection The ccdB gene located between the two attR sites for negative selection The Herpes Simplex Virus thymidine kinase polyadenylation signal for proper termination and processing of the recombinant tran