基础研究骨库骨的抗原性比较研究.PDF

2001 9 14 9 China J Orthop T rauma, Sep 2001, Vol. 14, No. 9 533 王金成 陈伟 刘光耀 高忠礼 段德生 袁绍辉 张远鹰 ( , 130031) ! , 5 , 10 , , 87∀ 11 , ( P 005) 108 ∀ 10, 107 ∀ 14, 117 ∀ 07 138 ∀ 15 , , ( P 005) , , 139 ∀ 11( P 005) ! Comparative study on antigenicity of stored bone WA N G Jincheng, CH EN Wei , LI U Guangy ao, et al. ChinaJ ap an Union H osp ital of J ilin University Jilin Changchun , 130031) Abstract! Objective To study the ant igenicity of frozen, freezedried, decalcified and inorganic homo graft ones. Methods In the experiment, the average scaling time of skin of mice was used as the index to compare antigenicity of different kinds of stored one after they were grafted.T he mice w ere divided into 5 groups of 10 each: fresh homograft one group(group #) , frozen homograft one group( group ∃) , freeze dried homograft one group(group %) , decalcified homograft one group( group ) and inorganic homograft one group ( group ∋) . Skin graft group served as control group. Results T he average scaling time of skin in group # was 8.7∀ 1. 1 days,w hich w as much longer than that of control group( P 005) .The average scal ing times of skin in group ∃, %, and ∋ w ere 10. 8∀ 1.0, 10. 7∀ 1.4, 11. 7∀ 0. 7 and 13. 8 ∀ 1. 5 days, w hich were much longer than that of group # ( P 005) . Conclusion The results show ed t hat t he anti genicity of stored ones decreased after they were handled y a ove methods. But the antigenicity of stored ones w ere not etter than that of grafted skin except inorganic homograft ones. The inorganic homograft one has no antigenicit y and is suita le to e used as carrier. Key Words! Bone transplantation Transplantation immunology Skin transplantation , ( BALB/ C , C57 ) , , 20 ∀ 2g; ,