欧洲黑杨育种基因资源SSR多态性比较研究.PDF

林 业 科 学 研 究　2006, 19 (4) : 477～483 F ores t R esea rch 　　文章编号 : 100 11498 (2006) 04 047707 欧洲黑杨育种基因资源 SSR多态性比较研究 张香华 , 苏晓华 , 黄秦军 , 张冰玉 (中国林业科学研究院林业研究所 , 国家林业局林木培育重点实验室 ,北京 　10009 1) ( ) 摘要 :本研究利用 13对 SSR 引物对 120份 105份国外引进 、15份我国新疆 欧洲黑杨基因资源进行了遗传多态性 分析 ,共检测出 17 1个等位基因 ,每个多态性位点检测到 7 ～19个等位基因 ,平均为 132 ,多态信息指数为 0. 396 ～ 0. 909,平均为 0808;遗传距离为 0 ～0456 4。欧洲黑杨基因种源具有丰富的遗传多样性 。通过 U PGMA 等类分析 , 将 120份材料划分为 8类 ,地理分布较近的材料基本聚在一起 ,表明遗传距离与地理距离相关性强 。 关键词 :欧洲黑杨 ;基因资源 ; SSR;遗传多样性 中图分类号 : S7223　　　　　文献标识码 : A Com por ison of Genetic Var ia tion s of B lack Poplar( P opu lu s n ig ra L. ) Gene Resource by M icrosa tellite M arker s ZHAN G X iang hua, SU X iaohua, HUAN G Q in j un, ZHAN G B ing y u (R esearch In stitu te of Forestry, CA F, Key L aboratory of Tree B reeding and Cu ltivation , State Forestry A dm in istration, B eij ing　100091, Ch ina) Ab stract: The simp le sequence rep eat ( SSR ) or m icro satellite m arker is currently p referred in genetic analysis due to its h igh ly de sirab le p rop ertie s. 13 p airs of SSR p rim ers were u sed to detect genetic diversity among 120 P op u lus n ig ra L. gene re source. A total of 17 1 allels were ob served among the polymorph ic loci, 7 ～19 SSR alleles were detected for each locu s w ith an average of 13. 2 alleles p er locu s. 13 p airs of SSR p rim ers were polymorph ic w ith the polymorph ism inform ation content ( P IC) ranging from 0. 396 to 0. 909. The 120 clones were clu stered based on the banding p attern s generated by 13 polymorp h ic SSR loci. In general, th is study indicate s that SSR s are sufficiently abundant and polymorph ic to be u sefu l ge netic m arkers in g