吡虫啉人工抗原的合成及鉴定.pdf

2005,38(3):511-515 Scientia Agricultura Sinica 1 2 310029 310012 µ· µ· µ· Synthesis and Identification of Artificial Antigen for Imidacloprid 1 1 1 1 2 ZHU Guo-nian , GUI Wen-jun , ZHENG Zun-tao , CHENG Jing-li , WU Gang 1 ( Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029; 2 Zhejiang Exit-Entry Inspection and Quarantine Bureau, Hangzhou 310012 ) Abstract: This study reported that a hapten of imidacloprid, 1-[(6-Carboxylethylthio-3-pyridinyl)methyl]-N-nitro- imidazolidinimine was synthesized by using technical grade imidacloprid reacted withβ-Mercaptopropionic acid in the hot alkalic solution. The hapten was conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to form the artificial antigens, respectively. The antibody with high titer (2.56×104) was produced after immuning to rabbits. Results showed that the antibody hadhigh affinity to imidacloprid tested by enzyme linked immunosorbent assay (ELISA), which IC50 and IC20 were 20.7 µg·L-1 and 1.1 µg·L-1 ; and the crosss-reactibities to the related compounds wereless than 1.4%. Key words: Imidacloprid; Artificial antigen; Antibody [ imidacloprid1-6--3--N --2 ] 95% 99.7 β- Fluka BSAMW67 000 HPLC SIGMA