粘附分子CD11b及ICAM-1在PMNs跨内皮移行中的作用机制研究_百替生物.pdf

粘附分子CD11b 与ICAM-1 在PMNs 跨内皮移行中的作用机制研究 张雪萍 刘新波 任永功 石碧明 李亚丽 [摘要] 目的探讨粘附分子CD11b 与ICAM-1 在PMNs 跨内皮移行中的作用。方法培养原代人脐静脉内 皮细胞于3.0 μM 孔径大小聚碳酸膜上直至其长成单层，然后移入一种48 孔两室趋化板内，LPS 分别 刺激PMN 及HUVEC ，取其中一部分分别用CD11b 单抗、ICAM-1 单抗、CD11b ＋ICAM-1 单抗处理， PMN 移行率通过计数跨越内皮细胞的PMNs 数决定。结果 LPS 激活PMNs 和HUVECs 后，PMN 跨 内皮移行显著增加(168.77% ±3.60%,p0.01). ，CD11b 单抗和 ICAM-1 单抗可分别减少这种移行至 122.90%±4.65% 和114.90%±6.06% 。同样，合用两种单抗也近乎产生叠加效应(93.00% ±5.21%) 。结 论 粘附分子CD11b 与ICAM-1 的表达影响着中性粒细胞的跨内皮移行作用，且这两种分子有协同作 用。 关键词 CD11b ICAM-1 多形核白细胞 脐静脉内皮细胞 跨内皮移行 作者单位：暨南大学医学院附属第二医院深圳人民医院 本文受深圳市科技计划项目基金资助 编号 200204004 张雪萍 女 1969 年－ 博士研究生 副主任医师，研究方向：麻醉与危重病医学 Roles of adhesion molecules CD11b and ICAM-1 in PMN transmigrating across service@100 endothelium. ZHANG Xueping LIU Xinbo REN Yonggong SHI Biming LI Yali Department of Anesthesiology, ShenZhen people’s Hospital, the second subordinate hospjtal of JiNan Medical college, ShenZhen 518020, China [Abstract] Objective To investigate the roles of adhesion molecules CD11b and ICAM-1 in PMN transmigrating across endothelium. Method HUVECs were cultured on gelatin treated microporous membranes until an endothelial cell monolayer was grown. Then they were moved into a 48-well transwell chambers with polycarbonate membranes of 3.0 μm pore size system . PMNs were isolated from fresh heparinized blood of healthy volunteers by dextran sedimentation followed by Ficoll-HyPaque gradient centrifugation. PMNs and HUVECs were stimulated with LPS(100ng/ml),then treated or not with CD11bMoAb 、ICAM-1MoAb、 CD11bMoAb+ICAM-1MoAb. The rate of PMNs migration was measured by determining the number of PMNs through endothelial cell monolayers. Result There is an increase in the number of PMN transmigrating across endothelium monolayer following activation of the PMNs and HUVECs