北大神经生物学课件2绪论.pptVIP

Effect of HANS on heart rate of heroin addicts HANS治疗肥胖症：人体研究结果 大鼠实验结果（高能食物引起肥胖） Diet-induced Obesity (DIO) After 14 weeks 大鼠实验结果（体重差值） 大鼠实验结果（能量摄取） 大鼠实验结果（免疫组化） * Threshold (mA) of afferent fibers activated by EA (HANS) at different frequencies Aα 0 1 2 3 4 5 6 7 2 Hz 0.6 ms 15 Hz 0.3 ms 100 Hz 0.2 ms Types of nerve fibers * Threshold of excitation (mA) Aβ Aδ C Day of treatment 1 2 3 4 5 6 7 8 9 10 60 70 80 90 100 110 120 130 Control 2 Hz 2/100 Hz 100 Hz n=13-16 * * * * * * * * * Heart Rate (beat/min) Body Weight (kg) 0 1 2 3 4 5 6 7 8 9 10 11 Days of Treatment 45 50 55 60 Control 2Hz 2/100Hz 100Hz (n=26-32) Effect of HANS on Body Weight during Opiate Withdrawal * * * * * * * ** ** ** The dose of BPN needed by Heroin Addicts during detoxification BPN Dose (mg/d) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Days of Treatment (n=14) 0.0 0.5 1.0 1.5 2.0 2.5 ** ** ** ** ** Buprenorphine HANS + BPN ** P0.001, compared with BPN group CRAVING: the most difficult issue 一朝吸毒，终身想毒。 1 Month 0 50 100 Relapse Rate (%) * * * P0.05, compared with BPN group 92% 78% 57% 71% 100% 100% HANS Decreases Heroin Relapse Rate of the Heroin addicts 2 Month 3 Month BPN (n=12) HANS+BPN (n=14) * The auto-track conditioned place preference (CPP) instrument Morphine-induced conditioned place preference (CPP) Dose-dependent effect *p0.05, **p0.01, ***p 0.001; compared with 0mg/kg group Index of preference = TimeMor / (TimeMor +Timesaline) Morphine-induced CPP Linear dose effect relationship -2 -1 0 1 0 100 200 n=9-12 Lg of Mor Dose (mg/kg) difference ( s ) EA Suppression of CPP for Morphine Control Restrain Foot shock Needle 100Hz 2Hz 2/100Hz 0.0 2.5 5.0 7.5 10.0 *** *** Time Spent in the Drug-pairing Side (min) n=18~20 Effect of EA of Different Frequencies Effect of EA Reversed by Naloxone n=8~9 Needle 2/100Hz 2Hz 0.0 2.5 5.0 7.5 10.0 *** *** 2/100Hz +NX(5mg/kg) 2Hz +NX(1mg/kg) Reinstatement of morphine’s CPP on rats by stress Time Spent in the Drug-pairing Sid