HPLC和LC_MS_MS鉴定锁阳固精丸中非法添加的西地那非衍生物.pdf

HPLC 和 LC-MS/MS 鉴定锁阳固精丸中非法添加的西地那非衍生物 1 2 1 1 (1. 浙江省药品检验所，杭州 310004 ；2. 浙江工商大学食品与生物工程学院，杭州 310035) 摘要：目的 方法 -(HPLC-DAD) --(LC-ESI-MS/MS)结果 (hydroxyhomosildenafil)结论 -(LC-MS/MS) 关键词：-- 中图分类号：R917.101 文献标志码：B 文章编号：1007-7693(2010)02-0167-05 Identification of Sildenafil Analog Illegally Added in Dietary Supplement using HPLC and LC-MS/MS 1 2 1 1 XIANG Zhimin ，ZHANG Li ，ZHU Ming ，ZHENG Cheng (1.Zhejiang Institute for Drug Control, Hangzhou 310004, China; 2.Biotechnology Environmental Engineering, College of Food Science, Zhejiang Gongshang University, Hangzhou 310035, China) ABSTRACT: OBJECTIVE To establish a method based on mass spectrometry structural assignments for identification of sildenafil analog added illegally in herbal product. METHODS A high-performance liquid chromatography-diode array detection (HPLC-DAD) method and a liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method were applied. RESULTS Identification of hydroxyhomosildenafil, an analog of sildenafil was achieved by comparing UV spectrum and mass fragmentation results of the samples with those of sildenafil. Three batches of Suoyang Gujing Wan, a dietary supplement, were found containing hydroxyhomosildenafil. CONCLUSION Based on structural assignments of mass fragments, analog of sildenafil could be rapidly and accurately determined in herbal product. KEY WORDS: sildenafil analog; hydroxyhomosildenafil; LC-MS/MS 西地那非(sildenafil ，商品名为万艾可)是一种 物，以逃避药品监督检验。由于这些衍生物是未 5 型磷酸二酯酶(PDE5)抑制剂，已被批准为用于治 经批准而合成生产的、目前还不能得到对照品， 疗男性