牛奶中的三聚氰胺.pdf

《乳业科学与技术》 2011 年第 1 期 （总第 146 期） 27 表面增强拉曼光谱法快速检测牛奶中的三聚氰胺 1 1 2 2 3 4 赵宇翔 ，彭少杰 ，赵建丰 ，胡耀铭 ，喻文娟 ，韩奕奕 （1 上海市食品药品监督所，上海 200233 ；2 复旦大学分析测试中心，上海 200433 ；3 上海交通大学分 析测试中心，上海 215125 ；4 农业部食品质量监督检验测试中心（上海），上海 200436 ） 摘 要：基于表面增强拉曼光谱技术的便携式拉曼光谱仪运用于牛奶收购、运输过程中三聚氰胺的快速 测定进行了研究。利用三聚氰胺位于 708 cm-1~714cm-1 的拉曼光谱特征峰及其强度，对三聚氰胺进行定 性及半定量的快速测定。检测限为 2.0 mg/L ，常见含氮化合物尿素、亚硝酸钠等物质对本快速测定方法 无干扰。对每个样品的检测时间从样品制备到结果显示一般只需 10 min。 关键词：表面增强拉曼光谱技术；便携式拉曼光谱仪；三聚氰胺；牛奶 中图分类号：TS252.51 文献标识码：A 文章编号：1671-5187(2011)01-0027-03 Surface Enhanced Raman Scattering for the Rapid Detection of Melamine in Milk 1 1 2 2 3 4 Zhao Yuxiang , Peng Shaojie , Zhao Jianfeng , Hu Yaoming , Yu Wenjuan , Han Yiyi (1 Shanghai Food and Drug Administration, Shanghai 200233, China; 2 Centre for Analysis and Measurement, Fudan University, Shanghai 200433, China; 3 Centre for Analysis and Measurement, Shanghai Jiao Tong University, Shanghai 215125, China; 4 Food Quality Supervision and Testing Center(Shanghai), Ministry of Agriculture, Shanghai 200436, China) Abstract: Based on the Surface Enhanced Raman Scattering(SERS) technology, portable Raman spectrometer was applied to rapid detection of Melamine contained in milk samples Employing the characteristic peak located at 708 cm-1~714cm-1 and their intensity, qualification and semi-quantification can be accomplished with the detection limit of 2.0 mg/L. No interference with the detection of Melamine has been found among common nitrogen compounds (urea, sodium nitrite). Process for each sample, from sample preparation to result indication, can be finished within 10 minutes. Key words: Surface Enhanced Raman Scattering(SERS) technology, portab