正交试验法优选夏黄颗粒处方药材的乙醇提取工艺.pdf

现代药物与临床 Drugs Clinic 第 28 卷 第 4 期 2013 年 7 月 • 519 • 正交试验法优选夏黄颗粒处方药材的乙醇提取工艺 胡 乔 1, 2, 3，廖茂梁2, 3 ，张铁军2, 3* ，肖瑞颖 1, 2, 3 1. 天津中医药大学，天津 300070 2. 天津药物研究院 释药技术与药物代谢动力学国家重点实验室，天津 300193 3. 天津药物研究院 中药现代制剂与中药质量控制技术国家和地方工程实验室，天津 300193 摘 要：目的 优选夏黄颗粒处方药材的乙醇提取工艺条件。方法 以乙醇体积分数、乙醇用量、提取时间、提取次数为考 察因素，采用 L 4 9(3 )正交试验设计，对 5 种蒽醌总转移率、出膏率两个指标进行综合评分，优选出最佳工艺。结果 最佳提 取工艺为 10 倍量 60%乙醇回流提取2 次，每次 1.5 h。结论 优选出的夏黄颗粒处方药材的最佳工艺合理，经验证其稳定可 行，有效成分提取率较高。 关键词：夏黄颗粒；总蒽醌；出膏率；正交试验 中图分类号：R284.2 文献标志码：A 文章编号：1674 - 5515(2013)04 - 0519 - 04 DOI: 10.7501/j.issn.1674-5515.2013.04.013 Optimization of ethanol extraction process for medicinal materials in Xiahuang Granule prescription by orthogonal test HU Qiao1, 2, 3, LIAO Mao-liang2, 3, ZHANG Tie-jun2, 3, XIAO Rui-ying1, 2, 3 1. Tianjin University of Traditional Chinese Medicine, Tianjin 300073, China 2. State Key Laboratory of Drug Delivery Technologies and Drug Metabolism, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China 3. The National and Local Engineering Laboratory of the Modernized Chinese Medicine and Chinese Medicine Quality Control Techniques, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China Abstract : Objective To optimize the ethanol extraction process for medicinal materials in Xiahuang Granule prescription. Methods Taking the concentration of ethanol, extraction time, amount of ethanol, and extraction times as factors, L 4 9(3 ) orthogonal test was designed and evaluated with multi-index of yiled and powdered extract yield of five anthraquinones. Results The optimal extraction technology was as follows: reflux extracted twice with 10 times the amount of 60% ethanol, for 1.5 h once. Conclusion This optimized ext