流感禽流感实验室诊断及新技术新进展课件.ppt

为什么流感病毒适用于NGS H3N2, H13N9 H3N8,H7N H3N2 , H4N5 7 H1-H16, N1-N9 H3N8 H1N1, H2N2, H3N2, H5N1, H7N7, H9N2,H10N7 H4, H5, H6,H7,H9, H10 ,H7N9 N1,N2,N4,N7 H5N1, H7N7, H9N2, H7N9 CHINESE CENTER FOR DISEASE CONTROL AND PREVENTION H4N6 H1N1, H1N2, H2N3, H3N2, H4N6 CHINESE CENTER FOR DISEASE CONTROL AND PREVENTION 利用CoverageAnalysis插件可以进行禽流感分型 Subtype: H9N2 Subtype: H7N9 CHIESE CENTER FOR DISEASE CONTROL AND PREVENTION H7N9和H9N2混合感染（培养样品） H7N9和H9N2混合感染（环境样品） 环境样品及其培养样品的比较：环境样本也能很好 地分型 ? 《Clinical and epidemiological characteristics of a fatal case of avian infl uenza A H10N8 virus infection : a descriptive study》 Timeline of the clinical course of the patient and identification of causative pathogen Frequency of glutamic acid was 87.6% versus 12.4% for lysine in the sample obtained on day 7, and frequency was 3.3% versus 96.7% on day 9. 深度测序发现PB2的第627位的杂合突变Glu/Lys,其与病毒感染哺乳动物 相关；在治疗过程中，Lys的突变比例增高对应其逐渐增强的毒力 CHINESE CENTER FOR DISEASE CONTROL AND PREVENTION (Virus) Evolution 进化生物学是研究生命的起源及进化的过 程、原因、机制、速率和方向的科学 起源（Origin）：从哪里来？ 进程（Process/Route）：怎么来的？ 原因（Causes）：为什么会来？ 机制（Selection）：谁驱动它？ 速率（Dynamics）：快？慢？ 方向（Direction）：要去哪？ Evolution of Evolutionary Methods 简单分子 数据 复杂分子 数据 简单个体 样本 复杂群体 样本 Phylogenetics Intra-host Variations Coalescence ??? 2013 H7N9 Avian Influenza Virus ? February 26, the first human infection ? March 31, identification of H7N9 ? April 11, the first publication on the New England Journal of Medicine ? China CDC, et al. ? H7N3 virus from ducks of Zhejiang ? H7N9 virus from wild birds flying across Korea ? Internal genes from H9N2 virus The Origin of H7N9 Origin and Route 1：The HA and NA genes were most probably transmitted from wild birds to domestic birds and then to humans 2：The six internal genes were from at least two distinct H9N2 virus populations of poultry 3：The intermediate hosts were still unknown,