DArT 技术的原理和其在植物遗传研究中的应用.pdf

HEREDITAS (Beijing) 2009 4 , 31(4): 359―364 ISSN 0253-9772 综述 DOI: 10.3724/SP.J.1005.2009.00359 DArT 技术的原理及其在植物遗传研究中的应用 1 2 1 1 1 洪义欢 , 肖宁 , 张超 , 苏琰 , 陈建民 1. , 225009; 2. , 225000 DArT 是一种依赖于基因芯片杂交基础之上的分子标记技术, 由于其具有省时、高通量、低花费、无需 预知研究对象的DNA 序列等优点而被应用到植物遗传研究中。文章介绍了 DArT 技术的原理、特点、具体操 作方法及其目前在植物遗传研究中的应用。 分子标记; DArT 技术; 杂交 Principle of diversity arrays technology (DArT) and its applications in genetic research of plants 1 2 1 1 1 HONG Yi-Huan , XIAO Ning , ZHANG Chao , SU Yan , CHEN Jian-Min 1. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China; 2. Agricultural Scicece Institute of Jiangsu Lixiahe District , Yangzhou 225000, China Abstract: Diversity arrays technology (DArT) is a microarray hybridization-based molecular marker technique, and a novel method to discover genetic polymorphic markers. DArT has recently been applied in genetic research of plants be- cause of its characteristics of sequence-independent, high–throughput, fast, low-cost. We briefly introduce the principle, characteristics, protocol of DArT and its application in genetic research of plants. Keywords: molecular markers; diversity arrays technology; hybridization 20 , DNA (Randoml amplified polymorphic DNA, RAPD), (Amplified fragment length ; ; polymorphism, AFLP), (Simple se- , ; quence repeat, SSR) ; RFLP ();