EMS诱变六倍体小麦偃展4110的形态突变体鉴定及分析.pdf

中国农业科学 2009,42(3):755-764 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2009.03.001 EMS诱变六倍体小麦偃展4110的形态突变体鉴定与分析 赵天祥，孔秀英，周荣华，高双成，贾继增 （中国农业科学院作物科学研究所/农业部作物种质资源与生物技术重点开放实验室，国家农作物基因资源与基因改良重大科学工程，北京 100081 ） 摘要： 【目的】构建小麦EMS突变体库，为小麦功能基因组学研究准备基础材料。【方法】利用化学诱变剂 甲基磺酸乙酯（ethyl methane sulfonate，EMS）诱变处理小麦品种偃展4110种子，将获得的M 代材料进行生物 2 学性状与农艺性状鉴定，部分M 材料播种家系进行验证。【结果】对M 代全生育期田间表型进行观察鉴定，突变 3 2 群体的表型变异率约为6.6%；获得了幼苗、叶、茎、穗及成熟期等生物学特性与主要农艺性状的变异体和突变体， 变异类型丰富，特别是发现了自然突变中少见的变异类型，如株高在10-15 cm左右的特矮变异类型。【结论】本 研究所构建的两个偃展4110 EMS突变群体较为理想，可望有效地被用于小麦功能基因组研究和小麦遗传改良中。 关键词：小麦；甲基磺酸乙酯(EMS)；突变体 Morphological Identification and Analysis of EMS Mutants from Hexaploid Wheat Cultivar Yanzhan 4110 ZHAO Tian-xiang, KONG Xiu-ying, ZHOU Rong-hua, GAO Shuang-cheng, JIA Ji-zeng (Key Laboratory of Crop Germplasm Biotechnology, The National Key Facility for Crop Gene Resources and Genetic Improvement, MOA/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081) Abstract: 【Objective 】 The mutant library is the basic material of functional genomics. In wheat, this kind of mutant library was rarely reported. In this study, two wheat mutant libraries with EMS were constructed for laying a foundation for wheat functional genomics research. 【Method 】 EMS was used as chemical mutagen to build the mutant library with early mature spring wheat Yanzhan 4110. Biological characters and agronomic traits were investigated in M2 generation. Some of mutants identified from M2 plants will be validated through M generation. 【Result 】 During the growth period after sowing the M seeds, al