RAD-seq技术在基因组研究中的现状和展望.pdf

Hereditas (Beijing) 2014 年 1 月, 36(1): 41 ―49 综 述 RAD-seq 技术在基因组研究中的现状及展望 王洋坤, 胡艳, 张天真 南京农业大学, 作物遗传与种质创新国家重点实验室/教育部杂交棉创制工程研究中心, 南京210095 摘要 : Restriction-site associated DNA sequencing(RAD-seq)技术是在二代测序基础上发展起来的一项基于全基 因组酶切位点的简化基因组测序技术。该方法技术流程简单, 不受有无参考基因组的限制, 可大大简化基因组 的复杂性, 减少实验费用, 通过一次测序就可以获得数以万计的多态性标记。目前, RAD-seq 技术已成功应用于 超高密度遗传图谱的构建、重要性状的精细定位、辅助基因组序列组装、群体基因组学以及系统发生学等基因 组研究热点领域。文章主要介绍了 RAD-seq 的技术原理、技术发展及其在基因组研究中的广泛应用。鉴于 RAD-seq 方法的独特性, 该技术必将在复杂基因组研究领域具有广泛的应用前景。 关键词 : RAD-seq; 基因组; 遗传图谱; SNP; 双酶系统的RAD(ddRAD)测序 Current status and perspective of RAD-seq in genomic research Yangkun Wang, Yan Hu, Tianzhen Zhang State Key Laboratory of Crop Genetics and Germplasm Enhancement/Cotton Hybrid R D Engineering Center of the Ministre of Educa- tion, Nanj ing Agricultural University, Nanj ing 210095, China Abstract: The restriction-site associated DNA sequencing (RAD-seq) is a high-throughput sequencing technique de- veloped from the next-generation sequencing (NGS). This method can reduce the representation of the complex genome while mapping thousands of polymorphic markers with or without a reference genome. It has been extensively used for high-density genetic map construction, fine mapping of important genes, genome sequence assembly, population genomic research, as well as phylogenetic research and so on. Here, we introduce the technological principle and development of RAD-seq combined with the sequencing applications in various species. Due to its uniqueness, RAD-seq will have a wide application in genetic analysis of complex genomic research in the future. Keywords: RAD-seq; genome; genetic map; SNP; double enzyme system of RAD (double digest RAD ddRAD)