戊唑醇在小麦中残留分析方法的研究.PDF

安徽农业大学学报, 2011, 38(3):434-438 Journal of Anhui Agricultural University [DOI]CNKI：34 －1162/S.0927.027 网络出版时间：2011-04-19 9:27:45 [URL]/kcms/detail/34.1162.S0927.027.html 戊唑醇在小麦中残留分析方法的研究 1 2 2* 1 汤 婕 ，岳永德 ，汤 锋 ，花日茂 (1. 安徽农业大学资源与环境学院，合肥 230036 ；2. 国际竹藤网络中心，北京 100080) 摘 要：在建立的戊唑醇残留分析方法中，小麦样品用丙酮为萃取溶剂，加水 10 mL，机械振荡法提取，提取 液用石油醚液液分配 2 次，中性氧化铝层析柱净化，气相色谱（GC-NPD ）检测。小麦中戊唑醇的添加浓度分别为 0.025 、0.05 和 0.5 mg·kg-1 ，其回收率分别为73.98% ～82.86%、81.21% ～95.38%和 81.51% ～97.98% ，变异系数为 5.74% ～9.33%。结果满足农药残留分析的要求。 关键词：戊唑醇；小麦；气相色谱法（GC ）；农药残留；检测 中图分类号：X592 文献标识码：A 文章编号：1672−352X (2011)03−0434−05 Method for residue analysis of tebuconazole in wheat with GC 1 2 2 1 TANG Jie , YUE Yong-de ,TANG Feng , HUA Ri-mao (1. School of Resources and Environment, Anhui Agricultral University, Hefei 230036; 2. International Center for Bamboo Rattan, Beijing 100080) Abstract: A residue analysis method was established for Tebuconazole. After mechanical oscillating ex- tracted twice with acetone, impurities were removed twice by liquid-liquid partition. Wheat samples extraction was cleaned up through a neutral Al O column , and then concentrated to be ready for detection with GC-NPD. 2 3 0.025, 0.05 and 0.5mg/kg of Tebuconzole in spiked wheat were analyzed, and the recoveries were in the range of 73.98% to 82.86%, 81.21% to 95.38%, and 81.51% to 97.98%, respectively. Coefficients of variation ranged from 5.74% to 9.33%. They all met the need of the pesticide residue analysis. Key words: tebuconazole; wheat; GC; pesticide residue; analysis 戊唑醇