枣花蕾总RNA提取方法的比较.PDF

第30 卷 第2 期 经 济 林 研 究 Vol. 30 No.2 2012 年6 月 Nonwood Forest Research Jun. 2012 枣花蕾总RNA提取方法的比较 冯延芝，袁德义，张 琳，龙洪旭，许靖诗，刘 敏 （中南林业科技大学 经济林育种与栽培国家林业局重点实验室，湖南 长沙 4 10004 ） 摘 要：为了探究枣花蕾总RNA 提取的最优方法，以沾化冬枣的花蕾为材料，利用改良CTAB 法、Trizol 法和 2 种RNA 提取试剂盒（PureLinkTM RNA 小提试剂盒和北京奥莱博生物技术有限公司提供的植物RNA 提取试剂盒） 分别提取花蕾的总RNA ，并对RNA 浓度和电泳图谱进行比较分析，利用奥莱博生物技术有限公司提供的植物 RNA 提取试剂盒法可提取到纯度较高，完整性好的总RNA ，并可用于Northern 杂交、cDNA 文库构建和半定量 RT-PCR 等分子生物学试验。 关键词：枣花蕾；RNA 提取；改良CTAB 法；Trizol 法；RNA 提取试剂盒法 中图分类号： S665.1 文献标志码：A 文章编号：1003—8981(2012)02—0088—03 Comparison of different extraction methods of total RNA from Ziziphus jujuba flower bud FENG Yan-zhi, YUAN De-yi, ZHANG Lin, LONG Hong-xu, XU Jing-shi, LIU Min （The Key Lab. of Non-wood Forest Product of Forestry Ministry, Central South University of Forestry Technology, Changsha 4 10004, Hunan, China ） Abstract: In order to study the optimal extraction method of total RNA from Ziziphus jujuba flower bud, using flower buds in Z. jujuba as materials, total RNA from Z. jujuba flower bud was extracted through improved CTAB method, Trizol method and 2 kinds of RNA extraction kit (Invitrogen PureLinkTM RNA Mini kit and RNA extraction kit from the Beijing Ao LaiBo Biological Technology Company). RNA concentration and electrophoregram were analyzed. The results proved that the method of RNA extraction kit from the Beijing Ao LaiBo Biological Technology Company could extract high-purity and highly-complete RNA, and it could be used in the molecular biology experiments, such as Northern hybridization, cDNA library establishment and s