octet生物分子相互作用仪.ppt

Multiple Systems at Top Pharma and Biotech Octet Academic and Government Customers Center for Disease Control Emory University Erasmus Medical Center Fachhochschule Nordwestschweiz Basel Florida Gulf Coast University Institute of Basic Medical Sciences Academy International AIDS Vaccine Institute Jeonnam Institute Kagoshima University Kennesaw State University Korea University NIH Penn State University Scripps Florida St. Jude University Stanford University Texas AM University TU Darmstadt Universit?t Würzburg University of Cambridge University of Florida University of South Florida University of Michigan University of Pittsburgh Cancer Institute Upstate Medical College MD Anderson Cancer Center Van Andel Institute Vanderbilt University Vienna Institute of Biotechnology Xiamen University Study Liposomes and Virus Like Particales Mutant 2? Full Analysis With 3 Conc Screening (20nM only in duplicate) ka (1/Ms) 2.56E+05 2.73E+05 kd (1/M) 2.41E-05 2.59E-05 Kd (M) 9.41E-11 9.49E-11 Mutant 1? Full Analysis With 3 Conc Screening (20nM only in duplicate) ka (1/Ms) 2.84E+05 3.21E+05 kd (1/M) 8.59E-05 9.46E-05 Kd (M) 3.02E-10 2.95E-10 Easily Determine Full Kinetics Characterization Data provided by Genitope Matrix Buffer Ka Kd KD 50% Plasma 5.20E6 7.91E-6 1.52E-12 PBS 4.39E6 6.63E-6 1.51E-12 Octet QK Association 1500 ng/mL Ag 1130 ng/mL Ag 760 ng/mL Ag 390 ng/mL Ag 205 ng/mL Ag 113 ng/mL Ag 20 ng/mL Ag Test Kinetics of Ab / Ag interaction Directly in Plasma What are Liposomes/VLPs? Liposomes (virus like particles-VLPs) are tiny “bubbles” (vesicles) made out of the same material as the cell membrane. They are not naturally occurring. They are made of phospholipids, with a hydrophilic (water-soluble) head, and hydrophobic (lipid-soluble) tail Size varies, typically from 10-150 nm in diameter Example Workflow to study VLP’s on the Octet Data analysis flexible to accommodate inverted and non-inverted data Donkey anti-mouse antibody binding Mouse Anti-CXCR4 antibody captu