四种核酸荧光染料在琼脂糖凝胶中染色特性的比较分析_张全波.pdf

四川解剖学杂志2007 年　第15 卷　第3 期 29 张全波, 冉黎, 陆长青, 陈红, 王凡* (四川大学华西医学中心基础与法医学院人体解剖学教 室, 成都610041) 　　【】　　(Genefinder、Geneview、S BER greenI) DNA / RNA (EB)。 RNA /DNA 。 RNA /DNA (P 0.05)。 Genefinder、Geneview S BER greenI EB, DNA / RNA 。 【】　;;Genefinder;Geneview;S BER greenI 【】　R737.9　　　【】　A A ComparativeAnalysison StainingEff Icienciesof FourFluorescent DyesinPreparativeAgarose GelElectrophoresis * Z HA N G Quan-bo, RA N Li , L U Chang-qing, CHEN Hong , WA N G Fan (Dep artment of Anatomy , Si huan University ,Chengd u 610041) 【Abstract】　Objective　To investigate the staining effect of Genefinder,Geneview and S BER gre- enI to the DNA /RNA samples in the gels and evaluate the possible application of them in routine nucleic acid staining as a substitutefor Ethidium Bromide(EB). ethods Comparative analysis was performed on the RNA /DNA staining efficiencies of four fluorescent dyes in preparative agarose gel electrophoresis.Results　There was no obvious difference among staining efficiencies of four fluo- rescent dyes (P0.05). Conclusion　The new nucleic stains of Genefinder, Geneview and S BER greenI may substitute for EB as general dyestuff to stain RNA /DNA in the gel. 【KeyWords】　Ncleic acid stain;Ethidium bromide;Genefinder;Geneview;S BER greenI 　　, 1　 , 、 、, 1. 1　 Genefinder S BER 。, greenI(, , ),Ge- 。, neview(, , ), EB 、、, (sigma, USA)。(6×, , , 。EB(Ethidium )。DNA Maker(Tiangen,, )。 Bromide,), Agarose gels (Oxoid, England)。DNA /RNA 、、, 。。 ,