染色体步行PCR技术.pdf

2006, 12( 3): 427~ 430 Ch in J App l E n vir on B io l= I SSN 2006206225 * PCR * * 王闵霞 马欣荣 王天山 谭 红 ( 610041) . PCR , PCR PCR PCR PCRSON PCR, PCR . , PCR . RAPD , . , . PCR , . 2 26 ; ; ; CLC Q78 * PCR Techn iques for Chrom osom eW alking * * WANGM i xia, MA Xi ro g, WANG Tia sha TAN H o g (Ch engdu Institute of Biology, Ch in ese Acad emy of Scien ces, Che gdu 610041, Ch i a) Abstract Ch romosom e walk i g tech iques are commo ly used for clo i g the k ow fla ki g seque ce. Th is paper reviews the existi g chromosom e walk i g PCR tech iques, such as i verse PCR, pa ha d le PCR, ligatio med iated (LM) PCR, ther2 m al asymm etric i terlaced ( TA IL) PCR a d si gle oligo ucleotide ested (SON) PCR, a d also puts forward a ew chromo2 som e walki gm ethod. Usi g th ism ethod the objective products are amplified u der commo PCR proceed i gw ith particu lar prmi er a d ra dom prmi er. Compariso expermi e tsw ith correspo d i g ra dom prmi er pairs are co ducted to ide tify the o 2 objective products. Thism ethodm ay be a ew a d effective tech ique for chromosom e walk i g. Fig 2, Ref26 K eyword s chromosom ewalk i g; particular prmi er; ra dom prmi er; ew tech ique