拟南芥LeafyCotyledon2的表达提高了小球藻.PDF

生 物 工 程 学 报 刘长斌 等/拟南芥Leafy Cotyledon 2 的表达提高了小球藻Chlorella sorokiniana 的油脂含量 1037 Chinese Journal of Biotechnology /cjbcn June 25, 2017, 33(6): 1037−1045 DOI: 10.13345/j.cjb.160462 ©2017 Chin J Biotech, All rights reserved ·生物技术与方法· Leafy Cotyledon 2 Chlorella sorokiniana 刘长斌，佟少明，张文蕾，侯和胜 116000 , , , . Leafy Cotyledon 2 Chlorella sorokiniana . , 2017, 33(6): 1037–1045. Liu CB, Tong SM, Zhang WL, et al. Expression of Leafy Cotyledon 2 from Arabidopsis increased the content of lipid in Chlorella sorokiniana. Chin J Biotech, 2017, 33(6): 1037–1045. 摘 要: 油脂含量是影响微藻产业化生产生物柴油的因素之一。基因工程的方法是培育高产藻株的一个重要 手段。Leafy Cotyledon 2 (LEC2) 在拟南芥中是调节种子成熟及油脂积累的重要转录因子，在藻类植物中尚无 相关的报道。本研究从拟南芥中获取LEC2 基因，构建表达载体pCIMBIA1300-35s-GFP-ATLEC2 ，通过基因枪 介导法转入小球藻 C. sorokiniana。经过PCR 、RT-PCR 、Western blotting 分析，筛选出一株转ATLEC2 基因藻 株。对总脂肪酸含量的分析发现，转ATLEC2 基因藻株的脂肪酸含量比原始藻株提高了 1 倍且没有明显影响生 长。以上结果表明，ATLEC2 能促进小球藻油脂的积累。 : 小球藻，转录因子，Leafy Cotyledon 2 ，油脂 Expression of Leafy Cotyledon 2 from Arabidopsis increased the content of lipid in Chlorella sorokiniana Changbin Liu, Shaoming Tong, Wenlei Zhang, and Hesheng Hou Liaoning Key Laboratory of Plant Biotechnology, Liaoning Normal University, Dalian 116000, Liaoning, China Abstract: The low lipid content is one of the major bottlenecks to realize the industrialization of the algae biodiesel. Improvement of lipid content through global regulation to get high-yield generating algae is a good strategy. Leafy Received: November 30, 2016; Accepted: April 11, 2017 Corresponding authors: Changbin Liu. Tel: +86-411 E-mail: 316093032@ Hesheng Hou. Tel: +86-411 E-mail: hesh