利用局部垂直切片与转距测量法研究生精小管内支持细胞核的位置与体积_杨正伟.pdf

1999 9 4 3 CHINESE JOURNA OF STEREO OGY AND IMAGE ANA YSIS Vol. 4, No. 3 Sept 1999 # 137 # * ( 川北医学院形态定量研究室 南充 637007) = 从 ( 1) 正常对照及经庚酸睾酮治疗了21 周的成年白人, 以及 (2) 正常对照及经 GnRH 拮抗剂治疗了16~ 25d 的成年猕猴, 获取25Lm 厚甲基 烯酸树脂包埋的睾丸组织切片 首先用无偏体视学新工具) 光学体视框, 在生精小管壁的局部垂直切片均匀抽选支持细胞核 ( 通 过抽选其核仁) , 然后( 1) 直接测量从所抽核仁中心或细胞核外侧 ( 基膜) 端至生精小管基膜内 侧缘之间的最短直线距离, ( 2) 并利用无偏体视学新方法) 垂直转距测量法测量所抽细胞核的体 积根据是否在生精周期上半部分 (人的第Ñ~ Ò期, 猴的第Ñ~ Ö期) 将支持细胞分成两 个时 相结果显示, 正常猴支持细胞核在上半生精周期离基膜的距离显著远于下半周期, 这提示支持 细胞核随大量生精细胞从基底向管腔的发生而移动但在人未检出这种周期性改变, 这可能与人 生精细胞在管壁内的电螺旋状 (非节段性) 分布有关上半和下半生精周期相比, 人或猴支持细 胞核的平均体积都无显著差异, 但用上述治疗显著抑制精子发生后支持细胞核的平均体积都显著 缩小约22~ 34% 人 猴 睾丸 生精小管 支持细胞 局部垂直切片 光学体视框 转距测量法 体视学 Local vertical setion and rotator measurement for evaluating the position and volume of Sertoli cell nuclei in seminiferous tubules Yang Zheng w ei ( Morphometric Research aboratory, North Sichuan Medical College, Nanchong 637007) Abstract 25Lm- thick methacrylate embedded tesicular sections were obtained from ( 1) control and testosterone enanthate-treated (for 21 weeks) men ( Caucasian) , and ( 2) control and GnRH antago- nist-treated ( for 16~ 25 days) adult monkeys ( Macaca fascicularis) . Sertoli cell nuclei were first uni- formly sampled from local vertical sections of the seminiferous tubules by samling their nucleoli using a contemporary unbiased stereological too-l the optical disector. then, ( 1) the distance from the center of the nucleolus and the nearest distance from the outermost end of the nucleus to the inner side of the basement membrane of the tubule were directly measured, and ( 2) the volume of each nucleus was measured with a contemporary