内毒素、前列腺素、白介素及炎症的关系研究Characterisation of the rapid release of activin A following.pdf

69 Characterisation of the rapid release of activin A following acute lipopolysaccharide challenge in the ewe 1 2 K L Jones, D M de Kretser, I J Clarke , J-P Y Scheerlinck and D J Phillips Monash Institute of Reproduction and Development, Monash University, Clayton, Victoria, Australia 1Prince Henry’s Institute of Medical Research, Clayton, Victoria, Australia 2Centre for Animal Biotechnology, The University of Melbourne, Victoria, Australia (Requests for offprints should be addressed to D J Phillips, Centre for Molecular Reproduction and Endocrinology, Monash Institute of Reproduction and Development, 27-31 Wright Street, Clayton 3168, Victoria, Australia; Email: david.phillips@.au) Abstract A series of experiments were conducted in adult ewes to blocking prostaglandin synthesis. Flurbiprofen, a prostag- delineate the release profile of activin A and its relationship landin synthesis inhibitor, effectively attenuated the fever to other cytokines following an i.v. injection of the response to LPS and partly inhibited cortisol release, but bacterial cell wall component, lipopolysaccharide (LPS). the cytokine profiles were unaffected. Finally, the bioac- Following this challenge, plasma activin A increased rap- tivity of TNF and/or IL-1 was blocked using soluble idly and appeared to be released in a biphasic manner, receptor antagonists. These treatments did not affect slightly preceding the release of tumour necrosis factor- the initial release of activin A, but blockade of TNF (TNF) and before elevation of interleukin (IL)-6 and depressed the second activin peak. These studies define follistatin levels. The concentration of activin A was