睾丸间质细胞的分离和培养体会.pdf

2008 40 7 # 21# 猪睾丸间质细胞的分离及培养 黄雅琼, 杨素芳, 农 微, * 谢体三, 谭世俭, 石德顺 (, 530005) : , , , 4~ 6 h ; 48 h , ; 3~ 5 d : ; ; ; : S852116 : A : 0529-5130( 2008) 07-0021-05 Isolation and in vitro cu lture of porcine leydig cell HUANG Ya-qiong, YANG S -fang, NONG W ei, * X IE T-i shan, TAN Sh-i jian, SH IDe-sh n ( Instit te ofAn mi al Reprod ction, G angxiUn iversity, N ann ing 530005, Ch ina) Ab stract: In order to enrich the donor variety of the som atic cell n clear transfer, m ethods for isolation and c lt re of porcine testis leyd ig cell w ere investigated in this st dy. Porcine testis leyd ig cells isolated by enzymatic digestion and steel net filtrationw ere ro nd and large. It began to attach cl mps after 4~ 6 h of c lt re, proliferated to form monolayers at 48 h of c lt re, and confl ented on 3~ 5 d of c lt re. These res ltsw ill provide a basis for isolation and in v itro c lt re of porcine testis eydig cells. Key wo rds: porcine; leydig cells; dissociation; c lt re ( interstitial cell) leydig cel,l , : 2007-07-15 , : ( 863 ) ( 2005AA206130),