树突状细胞与基因缺陷腺病毒联合治疗小鼠前胃癌的实验研究_霍伟.pdf

·836 · 霍伟 ,等 　树突状细胞和基因缺陷腺病毒联合治疗小鼠前胃癌的实验研究 实验研究 树突状细胞和基因缺陷腺病毒联合治疗小鼠 前胃癌的实验研究 1 2 2 3 霍伟 , 　张阳 , 　冷松 , 　李志民 1. 大连市肿瘤医院肿瘤科 ,辽宁 大连 116033 2 . 大连医科大学第二附属医院肿瘤科 ,辽宁 大连 116023 3 . 大连大学附属中山医院肿瘤科 ,辽宁 大连 11600 1 Combined dendritic cells and genedeleted adenovirus therapy for murine forestomach carcinoma 1 2 2 3 H UO Wei , Z HA N G Yang , L E N G S ong , L I Z him i n 1. D ep a rt ment of Oncology , D al i an Cance r H osp i t al , D al i an 116033 , P. R . Chi na 2. Dep artment of Oncology , S econd Af f iliated Hosp ital of D alian Medical University , D alian 116023 , P. R. China 3. D ep a rt ment of Oncology , A f f i l i ate d Z hongs han H osp i t al of D al i an Uni ve rs i ty , D al i an 11600 1 , P. R . Chi na ( 【摘要】　目的:观察树突状细胞 den [ABSTRACT] 　OBJECTIVE : To evaluate the efficacy of combined dendritic ) ( ) dritic cell s ,DCs 和基因缺陷腺病毒联 cells DCs and genedeleted adenovirus therapy in murine model with mouse 合治疗小鼠前胃癌的疗效 。方法 :建立 forestomach carcinoma ( MFC) . METHODS : MFC model was established. 小鼠前胃癌动物模型 ,在体外分化 、诱 Primary DCs were obtained from mouse bone marrow precursors as described 导 DCs ;瘤内注射基因缺陷腺病毒 ,观 previously. Murine bone marrow cells were plated in complete RPMI1640. 察肿瘤坏死情况 ; 再在瘤体 内注射 DCs and genedeleted adenovirus were introduced either alone or in combination DCs ,观察给药侧瘤体及对侧瘤体体 into the right tumor by direct intratumoral inj ection , and the necro si