微小RNA206与连接蛋白43在乳腺癌原发灶及腋窝转移淋巴结中的表达变化.pdf

( ) 2011 2 5 1 Chin J Breast Dis(Electronic Edition) , February 2011, Vol. 5,No. 1 65 实验研究 RNA206 43 明佳 范林军 钟玲 陈庆秋 姜军 RNA 206 ( miR206) ( C ) 43 ( PTs) ( MLNs) 8 , PTs ( NLNs) MLNs, PCR Western blotting , PTsNLNsMLNs miR206 C 43 mRNA Post hoc ( SNK/ LSD) , PTs NLNs miR206 mRNA , MLNs miR206 mRNA PTs NLNs ( P 0. 05) PTs NLNs MLNs C 43mRNA ( P 0. 05) , PTs MLNs C 43 mRNA ( P 0. 05) PTs NLNs C 43 ( P 0. 05) ; PTs , NLNs C 43 ( P 0. 05) , MLNs C 43 ( P 0. 05) miR206 C 43 ; ; ; RNA206; 43 R737. 9 A Expressions of miR206 and Cx43 in primary breast tumours and metastatic lymph nodes MI N G J ia, FA N L inj un, ZH ON G L ing , CH EN Qingqiu, JIA N G J un . Breast Disease Center, S uthw est H sp ital, Third M ilitary M edical University, Ch ngqing 400038, China C rresp nding auth r : JIA N G J un, Email : j cbd@ medmail. c m. cn Abstract Objecti e T o e plore the e pression and regulation role of microRN A206( miR206) and conne in 43 ( C 43) in primary breast tumours ( PT s) and metastatic lymph nodes ( M LN s) . Methods Eight patients of pathologically confirmed infiltrating duct carcinoma of the breast w er e enrolled. Pericancer nor mal tissues, primary br east tumors ( PTs) , paired normal a illary lymph nodes ( NLNs) and pair ed metastatic lymph nodes ( MLNs) w ere obtained fr om the resected tumor s of the patients. The e pressions of mRNA and protein of miR206 and C 43 in per icancer normal tissue, PT s, NLNs and M LNs w ere tested using realtime PCR and Western blot techniques. Comparison betw een groups w as performed using one w ay analy sis of var iances and SNK or LSD test . Results Compared w ith the normal breast tissues, the mRN A e pression of miR206 in PTs and NLNs decreased significantly, and it further decreased in M LNs compar ed w ith PTs and NLNs ( P 0. 05) . The : ( 2008BB5102)