柚皮苷通过调控GSK-3β表达对糖尿病心肌病大鼠模型的心肌保护作用研究.pdfVIP

·264· 中国现代药物应用2015年6月第9卷第11期　Chin J Mod Drug Appl, Jun 2015, Vol. 9, No. 11 ·实验研究· 柚皮苷通过调控 GSK-3β 表达对糖尿病心肌病 大鼠模型的心肌保护作用研究 黄瑞娜  吴铿  游琼  李腾  李上海  郭润民  闫海 【摘要】 目的 明确柚皮苷是否通过调控糖原合成酶激酶 -3β(GSK-3β) 通路对抗糖尿病心肌 病心肌损伤。 方法 将 60 只大鼠随机分为对照组、糖尿病心肌病组 (DCM 组 )、柚皮苷组、 TDZD- 8(GSK-3β 抑制剂 ) 组 , 各 15 例。各组经处理后 , 检测血糖 , 超声心动图检测左心室功能参数 , 免疫 组织化学检测心肌组织 GSK-3β 表达。结果 实验第 12 周 , 与柚皮苷组、TDZD-8 组比较 , DCM 组血 糖升高 , 左心室舒张期末内径 (LVDD)、左心室收缩期末内径 (LVDS) 增大 , 左心室射血分数 (LVEF) 下 降 , 差异有统计学意义 (P0.05)。结论 柚皮苷在有效调节糖代谢的同时 , 下调 GSK-3β 表达而心功 能恶化。 【关键词】 糖尿病心肌病 ；柚皮苷 ；糖原合成酶激酶 3β ；心功能 DOI ：10.14164/j.cnki.cn11-5581/r.2015.11.181 Research of protective effect by naringin for myocardium in diabetic cardiomyopathy rats by regulating β HUANG Rui-na, WU Keng, YOU Qiong, et al. Department of Cardiovascular Medicine, expression of GSK-3 Affiliated Hospital of Guangdong Meidcal College, Zhanjiang 524001, China 【Abstract 】 Obj ective To explore the approach of naringin in prevention of myocardial damage in diabetic cardiomyopathy by regulating expression of Glycogen synthase kinase 3β (GSK-3β). Methods A total of 60 rats were randomly divided into control group, diabetic cardiomyopathy group (DCM group), naringin group, and TDZD-8 (GSK-3β inhibitor) group, and each group contained 15 cases. Detections were made on blood glucose, left ventricular function parameters by ultrasonic cardiogram, GSK-3β expression in myocardial tissue by immunohistochemical method. Results In the 12 weeks of experiment, DCM group had increased blood glucose, left ventricular diastolic diameter (LVDD), left ventricular diameter systolic (LVDS), and decreased left ventricular ejection fraction (LVEF) then the naringin group and TDZD-8 group. Their differences had 作者单位 ：524001 广东医学院附属医院心血管内科 通讯作者 ：吴铿 镁浓度。