HPLC测定复方抗结核凝胶中异烟肼和盐酸左氧氟沙星的含量.PDF

HPLC 测定复方抗结核凝胶中异烟肼和盐酸左氧氟沙星的含量 王雪明，熊晗晖，王亚男(解放军总医院第二附属医院药剂科，北京 100091) 摘要：目的 建立高效液相色谱法同时测定复方抗结核凝胶中异烟肼和盐酸左氧氟沙星的含量。方法 采用 C18 色谱柱， 流动相: 乙腈-0.05 mol·L1KH PO (18 ∶82)，检测波长: 262 nm，柱温：30 ℃。流速: 0.8 mL·min1。结果 异烟肼和盐酸 2 4 左氧氟沙星均在 2~20 μg·mL1 内线性关系良好( r=0.999 8 ) ，平均回收率分别为 99.38%和 100.12%。结论 本法简便、准 确，可用于该制剂的质量控制。 关键词：高效液相色谱法；异烟肼；盐酸左氧氟沙星；复方抗结核凝胶 中图分类号：R917.101 文献标志码：B 文章编号：1007-7693(2010)06-0540-03 Determination of Isoniazid and Levofloxacin in Compound Antituberculosis Gel by HPLC WANG Xueming ，XIONG Hanhui ，WANG Yanan(Department of Pharmacy, The Second Affiliated Hospital, General Hospital of PLA, Beijing 100091, China) ABSTRACT: OBJECTIVE To establish a method for simultaneous determination of isoniazid and levofloxacin in compound antituberculosis gel by HPLC. METHODS C column was used. Acetonitrile-0.05 mol·L1 KH PO (18 ∶82)was used as 18 2 4 mobile phase. The flow rate was 0.8 mL·min1 and the detection wavelength was set at 262 nm. The column temperature was 30 ℃. RESULTS Isoniazid and levofloxacin had good linear relationship in the range of 220 μg·mL1 (r=0.999 8). The average recovery rate of isoniazid and levofloxacin were 99.38% and 100.12%, respectively. CONCLUSION The method is simple, accurate. It is suitable for quantitative analysis of isoniazid and levofloxacin in compound antitubercubosis gel. KEY WORDS ：HPLC; isoniazid; levofloxacin; compound antituberculosis gel 作者简介：王雪明，女，副主任药师 Tel: (010E-mail: hanhuixiong@126.com ·540 · Chin JMAP, 2010 June, Vol.27 No.6 中国现代应用药学2010 年 6 月第27 卷第 6 期 近年来，由于结核病疫情回升，复治、难治 刻度，摇匀，用 0