MTT升级版细胞计数试剂盒CCK8说明书.pdf

Revised August 16, 2002 Cell Proliferation Assay and Cytotoxicity Assay Cell Counting Kit-8 Technical Manual Product Code: CK04-11 (1000 tests) CK04-13 (3000 tests) Contents General Information 1 Advantages 1 Storage 2 How to Use Cell Counting Kit-8 2 Required Equipments and Materials 2 Protocol 2 Cell Proliferation Assay 2 Cytotoxicity Assay 2 Background Control 2 Precautions 3 Frequently Asked Questions 3 References 3 Appendix 4 Cytotoxicity Assay Data 4 Product Code and Price 4 Related Products 4 General Protocol at a Glance 5 For the most up-to-date technical manual, access /tm GENERAL INFORMATION As shown in Figure 3, the absorbance of the CCK-8 assay solution is higher than that of MTT and Cell Counting Kit-8 (CCK-8) allows very convenient other tetrazolium salts that produce water-soluble assays by utilizing Dojindo’s highly water-soluble formazan dyes. Figure 4 shows that the cell tetrazolium salt. WST-8 (2-(2-methoxy-4-nitrophenyl)-3- proliferation assay using CCK-8 correlates well with 3 (4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, the [ H]-thymidine incorporation assay. Thus, the