绕丹宁-2-2.pdf

The rhodadyns™ a new class of small molecule inhibitors of dynamin GTPase 1 1 1 1‡ 1 2 Mark J Robertson, Gordana Hadzic, Joseph Ambrus, D Yuri Pomè, Emily Hyde, Ainslie Whiting, Anna Mariana,2 Lisa von Kleist,3 Ngoc Chau,2 Volker Haucke,3 Phillip J Robinson2 and Adam 1* McCluskey 1Chemistry, The University of Newcastle, University Drive Callaghan NSW 2308 Australia. Phone: +61249216486; Fax: +61249215472; Email: Adam.McCluskey@newcastle.edu.au 2Cell Signalling Unit, Children’s Medical Research Institute, The University of Sydney, Sydney, NSW 2145, Australia 3 Leibniz Institut für Molekulare Pharmakologie Freie Universität Berlin, 13125 Berlin, Germany. Supplementary Data Chemistry General: All aldehydes were purchased reagent grade from Maybridge Trevillett England and rhodanines from TCI Japan Reagent Chemicals Association and utilized without further purification. All organic solvents were distilled before use. Synthesis under microwave irradiation was carried out in CEM Discover microwave. Analytical thin layer chromatography was applied on Merck silica gel 60 F254 precoated aluminum plates. ¹H and 13C spectra were recorded on a Bruker Advance AMX 300 MHz spectrometer at 300.13 and 75.48 MHz respectively. Chemical shifts are relative to 0.5% TMS as internal standard. Mass spectra were recorded on a Shimadzu LCMS 2010 EV using a mobile phase of 1:1 acetonitrile–H O with 0.1% formic acid or 5 mmol ammonium acetate. Accurate mass analysis was performed using an 2 Applied Biosystems QSTAR XL mass spectrometer. The machine was calibrated with