热带假丝酵母ctpxa1基因缺失菌的构建与对长链二元酸积累.PDF

/ ctpxa1 237 Chinese Journal of Biotechnology /cjbcn February 25, 2017, 33(2): 237−246 DOI: 10.13345/j.cjb.160278 ©2017 Chin J Biotech, All rights reserved ctpxa1 250353 , , , . ctpxa1 . , 2017, 33(2): 237–246. Cheng C, Wang JQ, Wang TF, et al. Effect of ctpxa1 gene deletion in Candida tropicalis on long chain dicarboxylic acid accumulation. Chin J Biotech, 2017, 33(2): 237–246. : Pxa1p Saccharomyces cerevisiae Pxa2p β- Candida tropicalis 1798 PCR ctpxa1 C. tropicalis 1798-pxa1 RT-PCR ctpxa1 C. tropicalis 1798C. tropicalis 1798-pxa1 2.03 ctpxa1 C. tropicalis 1798-pxa1 144 h C. tropicalis 1798-pxa1 C. tropicalis 1798 10.3 g/LC. tropicalis 1798 94.3% : ctpxa1p Effect of ctpxa1 gene deletion in Candida tropicalis on long chain dicarboxylic acid accumulation Cheng Cheng, Junqing Wang, Tengfei Wang, Xiaohui Yang, and Ruiming Wang College of Biological Engineering, Qilu University of Technology, Jinan 250353, Shandong, China Abstract: Candida tropicalis uses alkanes and fatty acids to produce long chain dicarboxylic acids. However, the yield can be affected by β-oxidation in peroxisomes. Pxa1p was a membrane protein of Saccharomyces cerevisiae peroxisomes. Received: June 22, 2016; Accepted: December 6, 2016 Supported by: Shandong Province Independent Innovation and Achievement Transformation Project (No. 201422CX02602). Corresponding author: Ruiming Wang. Tel: +86-531 E-mail: ruiming3k@163.com (No. 201422CX02602) 238 ISSN 1000-3061 CN 11-1998/Q Chin J Biotech February 25, 2017 Vol.33 No.2 Pxa1p and Pxa2p form a dimer that is involved in transporting of long chain fatty acids into peroxisomes, but the sim