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Carbon nanotube–titanium hybrid material (CC 2011)Supplementary information.pdf

Carbon nanotube–titanium hybrid material (CC 2011)Supplementary information.pdf

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Carbon nanotube–titanium hybrid material (CC 2011)Supplementary information

Electronic Supplementary Material (ESI) for Chemical Communications This journal is © The Royal Society of Chemistry 2011 Electronic Supplementary Information A novel bifunctional protein supramolecule for construction of carbon nanotube-titanium hybrid material. a b,c b b d Ippei Inoue, Bin Zheng, Kiyoshi Watanabe, Yasuaki Ishikawa, Kiyotaka Shiba, Hisashi Yasueda, a Yukiharu Uraokab,c and Ichiro Yamashita b a Frontier Research Labs., Institute for Innovation, Ajinomoto Co., Inc., 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki-shi, Kanagawa 210-8681, Japan b Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan c CREST, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan d Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Koto, Tokyo 135-8550, Japan Supplementary materials, methods and results Construction and purification of CDT1 and DT To obtain the synthetic gene encoding CDT1, the DNA fragment was amplified by whole vector PCR using primers (5’-tttggatccg aattcgagct ccgtcg-3’ and 5’-tttggatcct tacgcatccg gaagtttgcg catttctaat ggagcttttc-3’) and pET20b-NHBP-LiDps plasmid 1 as a template. The pET20b-NHBP-LiDps plasmid contained the gene encoding Listeria innocua Dps fused with only NHBP-1 at the N-t

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