单细胞光纳米光电检技术简介--V1020.ppt

江苏瑞明生物科技有限公司 单细胞纳米光电同步检测技术 在生命科学领域的应用 新的应用？ 1 2 3 4 单细胞技术 研究案例 单细胞光电检测技术 5 We all start out as a single totipotent cell that is programmed to produce a multicellular organism. How do individual cells make those complex developmental switches? How do single cells within a tissue or organ differ? how do they coordinate their actions or go astray in a disease process? Why do seemingly identical cells respond differently to a drug? Why single cell? Single-nucleus sequencing 癌细胞：复杂的亚细胞群，意味着复杂的基因组以及复杂的拷贝数变异（CNV），单细胞测序让这些“复杂”变得“简单化”，从而“清晰化”。 通过对来自于同一基因组背景的100个单细胞分别测序，鉴定到了一个原始的基因扩增。 (Nicholas et al., Nature, 472, 2011) 单细胞组学分析（Single-cell “omics” analysis） 单细胞转录组学分析揭示不同单细胞基因表达的巨大差别: Alex等利用脂多糖(LPS)刺激髓系来源的树突状细胞(BMDCs)，观察到单细胞在mRNA表达丰度和剪切模式方面的巨大差别，鉴定了137个刺激应答基因。 (Alex, et al. Nature, 498, 2013) Single cell transcriptomes 单细胞表观遗传分析？Not yet ! Big challenge, but possible, … 单细胞组学分析（Single-cell “omics” analysis） 单细胞组学分析（Single-cell “omics” analysis） Cohen, Science, 322, 2008 Single-cell analysis heralds a new era that allows omics analysis, notably genomics, transcriptomics, epigenomics and proteomics at the single-cell level. It enables the identification of the minor subpopulations that may play a critical role in a biological process of a population of cells, which conventionally are regarded as homogeneous. It provides an ultra-sensitive tool to clarify specific molecular mechanisms and pathways and reveal the nature of cell heterogeneity. It also facilitates the clinical investigation of patients when a very low quantity or a single cell is available for analysis, such as noninvasive prenatal diagnosis and cancer screening, and genetic evaluation for in vitro fertilization. Within a few short years, single-cell analysis, especially whole genomic sequencing and transcriptomic sequencing, is becoming robust and broadly accessible, although not yet a routine practice. 研究单细胞分析的论文调查: 据不完全统计：2013年发表在Cell上的关于Single Cell Analysis的论文有47篇，发表在Nature上的有8篇，发表