重氮甲烷衍生_气相色谱法测定黄瓜中乙烯利残留量_董祺杰.pdfVIP

- , , * ( , 200235) : 黄瓜样品经盐酸-乙醇( 5+ 95) 溶液提取, 并离心后经活性炭小柱过滤净化, 所得净化液 中乙烯利 重氮甲烷进行衍生反应, 产物用HP- 1701 毛细管柱( 0. 32 m m 30 m, 0. 25 m) 分离, 用火焰光度检测器测定乙烯利重氮甲烷化乙烯利的质量浓度在0. 100~ 2. 50 m g L- 1 之间 峰 面积呈线性方法用于黄瓜样品中乙烯利的测定, 平均回收率为97. 3%, 相对标准偏差( n = 5) 为 4. 5% : 气相色谱法; 衍生化; 乙烯利; 重氮甲烷; 黄瓜 : O657. 7 : A : 1001-4020( 2011) 09-107 1-03 GC Determination of Residual Amount of Ethephon in Cucumber After Derivatization with Di azomethane * DONG Qi-jie ZHAO Qun-hong LI Yu (S hang ha i M unic ip al Food R esear ch I nst it ute, Shang hai 200235, Ch ina) T he ample of cucumber w a ex tracted w ith a m ixt ur e of HC-l C H O H ( 5+ 95) , and t he ex tr act Abstract: 2 5 obtained w a eparated by centr ifugation and pur ified by pa ing through activ ated car bo n column. T he purified et hephon in the olution after filtr ation wa deriv atized w ith diazomethane, and the derivatized pro duct wa u ed for GC analy i . H P-170 1 capillary column ( 0. 32 mm 30 m, 0. 25 m) w a u ed for epar atio n and flame phot ometric detector wa adopted in the deter minatio n. Linear relatio n hip between v alue of peak area and ma co ncentration of et hephon wa obtained in the rang e of 0. 100 - 2. 50 mg L- 1 . T he propo ed method w a applied to the determ inat ion of ethy lene in cucumber, giving averag e recov ery of 97. 3% and RSD ( n= 5) of 4. 5% . Keywords: G C; Der ivatization; Ethephon; Diazomethane; Cucumber 2-, , ; pH 3 , ,