人子宫内膜异位症纤维化动物模型建立.doc

人子宫内膜异位症纤维化动物模型建立 　　[摘要] 目的 采用子宫内膜异位症患者的子宫内膜组织建立纤维化裸鼠模型。 方法 收集子宫内膜异位症患者的子宫内膜组织（增殖期及分泌期），使用开腹方法将所取子宫内膜组织种植入BALB/c裸鼠盆腹腔。观察裸鼠子宫内膜病灶生长以及裸鼠生存情况，分别于种植后的第7、14天开腹探查，观察异位病灶的位置、大小及与周围组织的关系，并取部分组织进行病理验证。 结果 人子宫内膜异位症裸鼠模型成功建立，裸鼠腹部表面可见隆起的结节，质韧，与周围组织粘连，活动度差，开腹探查可见异位病灶大部分位于腹壁及膀胱旁，病理证实异位病灶可见子宫内膜样腺体结构，周围有基质细胞及大量炎症细胞浸润，可见纤维组织沉积。 结论 子宫内膜异位症患者的子宫内膜种植于裸鼠盆腹腔内，可成功建立异体子宫内膜异位症纤维化裸鼠模型，为其发生、发展机制及其恶性生物学行为的研究提供平台 [关键词] 子宫内膜异位症；不孕；盆腔疼痛；盆腔包块；动物模型 [中图分类号] R711.71 [文献标识码] A [文章编号] 1673-7210（2016）11（b）-0008-04 [Abstract] Objective To establish a fibrotic nude mouse model of human endometriosis by taking advantage of endometrial patients. Methods Stage proliferation and secretion of endometrial tissues in patients diagnosed as endometriosis were collected， thus a minilapamtomy was performed to transplant fresh human endometrial pieces into the peritoneum of BALB/c nude mice. The growth of endometrial disease lesion and survival situation in nude mice. The mice were killed at 7 and 14 d after transplantation to observe the location and size of the ectopic lesion and its relationship with the surrounding tissue. Part of the tissues were examined by histopathology. Results The human endometriosis model was successfully established， in which we could saw toughening lumps burged on the abdomen surface of nude mice， with the surrounding tissue adhesion， and mobility was poor. The most endometrial lesions located in nape and nexted to bladder were observed by through exploratory laparotomy. Pathology confirmed that endometrial glandular structure was surrounded by stromal cells and inflammatory cells infiltration， and it had fibrous tissue deposition in ectopic lesions. Conclusion A nude mouse model of endometriosis has been successfully established. It may provide an appropriate model for research of the mechanism of endometriosis. [Key words] Endometriosis； Infertility； Pelvic pain； Pelvic cyst； Animal model 子?m内膜异位症（endometriosis，EMs）是指子宫内膜腺体和基质异位到宫腔以外的部分，以卵巢、子宫直肠陷凹、子宫骶韧带等部位最常见，是妇科一种慢性的、良性的、