大孔吸附树脂分离纯化蛹虫草固体培养基中虫草素工艺_胡瑕.pdf

110 2012, Vol. 33, No. 06 食品科学 ※工艺技术 大孔吸附树脂分离纯化蛹虫草固体培养基中 虫草素工艺 1,2 1 1 1 1, 胡 瑕 ，谢红旗 ，罗 巍 ，夏志兰 ，刘东波 * ( 1.湖南农业大学园艺园林学院，国家中医药管理局亚健康干预技术实验室，湖南 长沙 4 10 12 8 ； 2. 国家柑橘改良中心长沙分中心，湖南长沙 4 10 128) 摘 要：比较D 10 1 、AB -8 、HP D - 10 0 、HP D -4 0 0 、HP D -50 0 、HP D -72 2 、DM 13 0 七种大孔吸附树脂对蛹虫草 固体培养基中虫草素的吸附与解吸性能，筛选出HPD- 100 树脂为最佳树脂，并确定HPD- 100 树脂吸附分离最佳工 艺条件：上样液质量浓度0 .6mg/mL 、上样流速3BV/h 、上样体积6BV ；解吸剂为体积分数2 5% 乙醇溶液、解吸 流速2BV/h 、解吸体积4BV 。根据此工艺条件，蛹虫草固体培养基粗提物经HPD - 100 树脂纯化后，虫草素产品纯 度可达14 . 1% ，较粗提物产品提高了8 倍多。 关键词：虫草素；蛹虫草；大孔吸附树脂 Separation and Purification of Cordycepin from Spent Solid Culture Medium after Harvesting Cordy cep s militaris Fruit Bodies by Macroporous Resin Adsorption 1,2 1 1 1 1, HU Xia ，XIE Hon g-qi ，LU O Wei ，XIA Zhi-lan ，LIU Don g-b o * (1. Sub-health Intervention Technology Laboratory, State Administration of Traditional Chinese Medicine, College of Horticulture and Landscape, Hunan Agricultural University, Changsha 4 10 128, China； 2. Changsha Subcenter of National Center of Citrus Improvement, Changsha 4 10 128, China ) 　　 Abstract ：The adsorption and desorption characteristics of macroporous resin s D 10 1, AB-8, HPD-100, HPD-400, HPD-500, HPD-722 and DM 130 for cordycepin from spent solid culture medium after harvesting Cordy cep s militaris fruit bodies were comparatively investigated. The results showed that HPD- 100 was the best resin for the purification of cordycepin from the spent culture medium. The optimal separation and pur